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首页> 外文期刊>RSC Advances >Synergistic delivery of bFGF and BMP-2 from poly(l-lactic-co-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications
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Synergistic delivery of bFGF and BMP-2 from poly(l-lactic-co-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications

机译：BFGF和BMP-2的协同递送来自聚（L-乳酸 - 共乙醇酸）/石墨烯氧化物/羟基磷灰石纳米纤维支架，用于骨组织工程应用

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摘要

One of the goals of bone tissue engineering is to create scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The application of bioactive proteins, such as bone morphogenetic protein (BMP)-2 and basic fibroblast growth factor (bFGF), has been showed to be an effective way to improve the osteoinductivity and biocompatibility of bone scaffold materials. Therefore, the development of novel materials capable of delivering multiple growth factors is urgent and essential for bone defect repair. In this study, a composite nanofibre scaffold composed of poly(l-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and graphene oxide (GO) has been fabricated to deliver basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) simultaneously. The data show that the incorporation of GO and HA into PLGA nanofibres significantly improved the mechanical properties and hydrophilicity of the nanofibre scaffolds. More importantly, compared to PLGA and PLGA/HA nanofibre scaffolds, the PLGA/HA/GO nanofibre scaffolds could more efficiently immobilize bFGF and BMP-2. Moreover, biological assays indicated that the loaded bFGF and BMP-2 loaded in the composite nanofibre scaffolds have a synergistic differentiation effect on the cell adhesion, proliferation, and osteogenesis differentiation of MC3T3-E1 cells. In contrast to the PLGA/HA/GO/bFGF and PLGA/HA/GO/BMP-2 nanofibre scaffolds, the PLGA/HA/GO/bFGF/BMP-2 scaffolds have shown higher ALP activity and higher expression levels of osteogenesis-related genes. In summary, our findings indicated that the incorporation of GO into nanofibre scaffolds is an effective method to immobilize growth factors onto biomaterial surfaces, and the synergistic effects of a combination of BMP-2 and bFGF may have potential use in bone regenerative therapeutics.
机译：其中的骨组织工程的目标是创建具有优良的生物相容性，骨诱导性和机械性能的支架。生物活性的蛋白质，如骨形态发生蛋白（BMP）-2和碱性成纤维细胞生长因子（bFGF）的应用，已经表明是改善的骨支架材料的骨诱导性和生物相容性的有效途径。因此，能够提供多种生长因子的新材料的发展是用于修复骨缺损的紧急和重要的。在这项研究中，复合纳米纤维支架由聚（L-乳酸 - 共 - 乙醇酸）（PLGA），羟基磷灰石（HA），和石墨烯氧化物（GO）已经被制造成提供基本成纤维细胞生长因子（bFGF）和骨形态发生蛋白-2（BMP-2）同时进行。数据显示，GO和HA掺入PLGA纳米纤维显著提高纳米纤维支架的机械性能和亲水性。更重要的是，相对于PLGA和PLGA / HA纳米纤维支架，所述PLGA / HA / GO纳米纤维支架可以更有效地固定bFGF和BMP-2。此外，生物测定表明，所装载的bFGF和BMP-2的复合纳米纤维支架加载有细胞粘附，增殖和成骨MC3T3-E1细胞分化的协同作用分化。与此相反的PLGA / HA / GO / bFGF和PLGA / HA / GO / BMP-2的纳米纤维支架，所述PLGA / HA / GO / bFGF的/ BMP-2的支架表现出更高的ALP活性和骨生成有关的更高的表达水平基因。总之，我们的研究结果表明，GO掺入纳米纤维支架是固定化的生长因子的生物材料上的表面，和BMP-2和bFGF的组合的协同效应可具有在骨再生治疗剂的潜在用途的有效方法。

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  • 来源
    《RSC Advances》 |2018年第56期|共13页
  • 作者

    Ren Xiansheng; Liu Qinyi; Zheng Shuang; Zhu Jiaqi; Qi Zhiping; Fu Chuan; Yang Xiaoyu; Zhao Yan;

  • 作者单位

    Jilin Univ Hosp 2 Phys Examinat Ctr Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 2 Dept Orthoped Surg Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 2 Dept Orthoped Surg Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 1 Dept Gynecol &

    Obstet Xinmin St 71 Changchun Tx 130000 Peoples R China;

    Jilin Univ Hosp 2 Dept Orthoped Surg Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 2 Dept Orthoped Surg Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 2 Dept Orthoped Surg Ziqiang St 218 Changchun Tx 130041 Peoples R China;

    Jilin Univ Hosp 2 Phys Examinat Ctr Ziqiang St 218 Changchun Tx 130041 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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