• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Nontargeted SWATH acquisition mode for metabolites identification of osthole in rats using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry
【24h】

Nontargeted SWATH acquisition mode for metabolites identification of osthole in rats using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry

机译：使用超高效液相色谱法耦合到四极其飞行时间质谱法的大鼠胞间孔鉴定的Nontargeted的SWATH采集模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Osthole (OST), 7-methoxy-8-isopentenoxycoumarin, is the characteristic constituent found in Cnidium monnieri (L.) Cuss. and possesses excellent pharmacological activities, including anticancer, anti-apoptosis and neuroprotection. In this study, a rapid and reliable method based on ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) and MetabolitePilot2.0 software with principal component variable grouping (PCVG) filtering was developed to observe probable metabolites of OST firstly. The high resolution mass data were acquired by data-independent acquisition mode (DIA), i.e., sequential window acquisition of all theoretical fragmentation spectra (SWATH), which could significantly improved the hit rate of low-level and trace metabolites. A novel data processing method key product ions (KPIs)' were employed for metabolites rapid hunting and identification as an assistant tool. A total of 72 metabolites of OST were detected in vitro and in vivo, including 39 metabolites in rat liver microsomes (RLMs), 20 metabolites in plasma, 32 metabolites in bile, 32 metabolites in urine and 37 metabolites in feces. The results showed that mono-oxidation, demethylation, dehydrogenation, sulfate conjugation and glucuronide conjugation were major metabolic reactions of OST. More significant, oxydrolysis, 3,4-epoxide-aldehylation, phosphorylation, S-cysteine conjugation and N-acetylcysteine conjugation were considered as unique metabolic pathways of OST, and phosphorylation, S-cysteine conjugation and N-acetylcysteine conjugation reactions were characterized in rat biological samples for the first time. Preparation of active metabolites will be greatly helpful in elucidating the potential biological mechanism of OST, and the proposed metabolic pathways of it might provide further understanding of the safety and efficacy of simple coumarins.
机译：Osthole（OST），7-甲氧基-8-异戊酚氧基苏林，是Cnidium Monnieri（L.）Cuss中发现的特征成分。并具有优异的药理学活动，包括抗癌，抗凋亡和神经保护作用。在本研究中，基于超高效液相色谱的快速可靠的方法，耦合到四极其飞行时间质谱（UHPLC-Q-TOF-MS）和MetabolitePilot2.0软件，具有主成分变量分组（PCVG）开发过滤以首先观察OST可能的代谢物。通过数据无关的采集模式（Dia），即顺序窗口获取所有理论碎片光谱（SWATH）的顺序窗口获取的高分辨率质量数据，这可以显着提高低水平和痕量代谢物的命中率。一种新的数据处理方法主要产品离子（KPI）'用于代谢物快速狩猎和识别作为辅助工具。在体外和体内检测到总共72个代谢物，包括大鼠肝微粒体（RLMS）的39代谢物，血浆中的20种代谢物，胆汁中的32个代谢物，尿液32个代谢物和粪便中的37个代谢物。结果表明，单氧化，去甲基化，脱氢，硫酸盐缀合和葡糖醛酸缀合物是OST的主要代谢反应。更重要，氧化溶解，3,4-环氧化 - 醛，磷酸化，S-半胱氨酸缀合和N-乙酰半胱氨酸缀合被认为是OST的独特代谢途径，并且在大鼠中表征了磷酸化，S-半胱氨酸缀合和N-乙酰半胱氨酸缀合反应生物样本是第一次。活性代谢物的制备将极大地有助于阐明潜在的OST生物机制，并且它可能提供了对简单香豆素的安全性和功效的进一步了解的拟议代谢途径。

著录项

  • 来源
    《RSC Advances》 |2018年第27期|共11页
  • 作者

    Liao Man; Diao Xinpeng; Cheng Xiaoye; Sun Yupeng; Zhang Lantong;

  • 作者单位

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal 361 East Zhongshan Rd Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal 361 East Zhongshan Rd Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal 361 East Zhongshan Rd Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal 361 East Zhongshan Rd Shijiazhuang 050017 Hebei Peoples R China;

    Hebei Med Univ Sch Pharm Dept Pharmaceut Anal 361 East Zhongshan Rd Shijiazhuang 050017 Hebei Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号