A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella

Gu Yingchun; Lin Dayong; Fei Xuening; Wang Cuihong; Yang Qi; Tang Yalin; Ren Xueling

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A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella

机译：一种新型荧光长链脂肪酸取代染料：MicroTrix Parvicella的标记和生物降解

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Microthrix parvicella (M. parvicella) is a filamentous bacterium that induces bulking in activated sludge. Here, we used the affinity of long-chain fatty acids (LCFA) for M. parvicella to create a novel fluorescent probe of carbazole modified by LCFA. The structure was characterized by H-1 NMR spectroscopy and mass spectrometry. The spectral properties, photostability, and hydrophobic properties of the probe were also characterized. Fluorescent-labeling results showed that it can label M. parvicella in situ and could be biodegraded via metabolism. The stable docking mode of carbazole probes with different fatty acid chains and lipases was also docked by the density functional tight-binding (DFTB) method.

机译：MicroTrix Parvicella（M. parvicella）是一种丝状细菌，可在活性污泥中诱导膨胀。在这里，我们使用了长链脂肪酸（LCFA）对M. parvicella的亲和力，以通过LCFA改性的新型咔唑的新荧光探针。该结构的特征在于H-1 NMR光谱和质谱。还表征了探针的光谱性质，光稳定性和疏水性质。荧光标记结果表明，它可以原位标记M. parvicella，可以通过代谢生物降解。用不同脂肪酸链和脂肪酶的咔唑探针的稳定对接模式也通过密度函数紧密（DFTB）方法对接。

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《RSC Advances》 |2018年第62期|共8页
作者
Gu Yingchun; Lin Dayong; Fei Xuening; Wang Cuihong; Yang Qi; Tang Yalin; Ren Xueling;
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Tianjin Chengjian Univ Tianjin Engn Technol Ctr Chem Wastewater Source R Sch Sci Tianjin 300384 Peoples R China;

Anda Duosen New Mat Technol CO LTD Anda 151400 Heilongjiang Peoples R China;

Tianjin Chengjian Univ Tianjin Engn Technol Ctr Chem Wastewater Source R Sch Sci Tianjin 300384 Peoples R China;

Tianjin Chengjian Univ Tianjin Engn Technol Ctr Chem Wastewater Source R Sch Sci Tianjin 300384 Peoples R China;

Tianjin Chengjian Univ Tianjin Engn Technol Ctr Chem Wastewater Source R Sch Sci Tianjin 300384 Peoples R China;

Chinese Acad Sci Inst Chem Beijing Natl Lab Mol Sci Beijing 100190 Peoples R China;

Zhengzhou Univ Key Lab Targeting Therapy &

Diag Crit Dis Sch Pharmaceut Sci Zhengzhou 450001 Henan Peoples R China;

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正文语种 eng
中图分类化学;
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1. A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella [J] . Gu Yingchun, Lin Dayong, Fei Xuening, RSC Advances . 2018,第62期

机译：一种新型荧光长链脂肪酸取代染料：MicroTrix Parvicella的标记和生物降解
2. A novel dicationic Quinoline-Carzole fluorescent probe: preparation and labelling of Microthrix parvicella [J] . Fei Xuening, Xing Yanjun, Zhang Buqing, Environmental Technology . 2020,第17a20期

机译：一种新型喹啉喹啉 - 胞氨酰型荧光探针：MicroTrix Parvicella的制备和标记
3. Synthesis and characterization of the fluorescent probes for the labeling of Microthrix parvicella [J] . Li Songya, Fei Xuening, Jiao Xiumei, Applied Microbiology and Biotechnology . 2016,第6期

机译：用于标记小蓟马的荧光探针的合成与表征
4. Cause and control of baulking sludge by Microthrix parvicella in MUCT system [C] . Jun Yin, Xiao-Ling Wang, Xiang-Hui Wu, The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集 . 2009

机译：MUCT系统中小百里香微囊菌堆积的污泥的原因及控制
5. Design, synthesis, and optimization of an optical sensing system utilizing boronic acid-substituted viologens and pyrene-based fluorescent dyes: Progress toward the development of an in vivo glucose monitor [D] . Gamsey, Soya 2006

机译：使用硼酸取代的紫精和-基荧光染料的光学传感系统的设计，合成和优化：体内葡萄糖监测仪的开发进展
6. Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix parvicella In Situ [O] . Xiumei Jiao, Xuening Fei, Songya Li, 2017

机译：新型荧光探针原位鉴定微丝菌的设计机理和性能
7. A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading ofMicrothrix parvicella [O] . Yingchun Gu, Dayong Lin, Xuening Fei, 2018

机译：一种新型荧光长链脂肪酸取代的染料：标记和生物降解的MiCrothrix Parvicella

A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella

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