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首页> 外文期刊>RSC Advances >A one-step fluorescent biosensing strategy for highly sensitive detection of HIV-related DNA based on strand displacement amplification and DNAzymes
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A one-step fluorescent biosensing strategy for highly sensitive detection of HIV-related DNA based on strand displacement amplification and DNAzymes

机译：基于链置换扩增和DNAzymes的高敏感检测高敏感检测的一步荧光生物腐蚀策略

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摘要

Sensitive and specific detection of HIV-related DNA is of great importance for early accurate diagnosis and therapy of HIV-infected patients. Here, we developed a one-step and rapid fluorescence strategy for HIV-related DNA detection based on strand displacement amplification and a Mg2+-dependent DNAzyme reaction. In the presence of target HIV DNA, it can hybridize with template DNA and activate strand displacement amplification to generate numerous DNAzyme sequences. With the introduction of Mg2+, DNAzyme can be activated to circularly cleave the substrate DNA, which leads to the separation of fluorophore reporters from the quenchers, resulting in the recovery of the fluorescence. Under the optimal experimental conditions, the established biosensing method can detect target DNA down to 61 fM with a linear range from 100 fM to 1 nM, and discriminate target DNA from mismatched DNA perfectly. In addition, the developed biosensing strategy was successfully applied to assay target DNA spiked into human serum samples. With the advantages of fast, easy operation and high-performance, this biosensing strategy might be an alternative tool for clinical diagnosis of HIV infection.
机译：敏感性和特异性检测HIV相关的DNA对于早期准确的诊断和治疗HIV感染患者的治疗非常重要。在这里，我们开发了一种基于链位移扩增的HIV相关DNA检测的一步和快速的荧光策略和MG2 +依赖性DNAzyme反应。在靶HIV DNA存在下，它可以与模板DNA杂交，并激活链位移扩增以产生许多DNAzyme序列。随着MG2 +的引入，可以激活DNAzyme以圆形切割底物DNA，这导致荧光团记者与猝灭剂的分离，导致荧光的回收率。在最佳的实验条件下，所建立的生物传感方法可以将靶DNA降至61 fm，线性范围为100 fm至1nm，并完美地区分来自非匹配的DNA的靶DNA。此外，已发发的生物强调策略已成功应用于测定靶向人血清样品的靶DNA。随着快速，操作简便和高性能的优点，这种生物凸版策略可能是艾滋病毒感染临床诊断的替代工具。

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  • 来源
    《RSC Advances》 |2018年第55期|共7页
  • 作者

    Yan Xiaoyu; Tang Min; Yang Jianru; Diao Wei; Ma Hongmin; Cheng Wenbin; Que Haiying; Wang Tong; Yan Yurong;

  • 作者单位

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Zunyi Med Univ Affiliated Hosp Dept Clin Lab Zunyi 563003 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

    Chongqing Med Univ Coll Lab Med Minist Educ Key Lab Clin Lab Diagnost Chongqing 400016 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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