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首页> 外文期刊>RSC Advances >Force spectra of single bacterial amyloid CsgA nanofibers
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Force spectra of single bacterial amyloid CsgA nanofibers

机译：单细菌淀粉样蛋白CSGA纳米纤维的力光谱

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摘要

CsgA is a major protein subunit ofEscherichia colibiofilms and plays key roles in bacterial adhesion and invasion. CsgA proteins can self-assemble into amyloid nanofibers, characterized by their hierarchical structures across multiple length scales, outstanding strength and their structural robustness under harsh environments. Here, magnetic tweezers were used to study the force spectra of CsgA protein at fibril levels. The two ends of a single nanofiber were directly connected between a magnetic bead and a glass slide using a previously reported tag-free method. We showed that a wormlike chain model could be applied to fit the typical force-extension curves of CsgA nanofibers and to estimate accordingly the mechanical properties. The bending stiffness of nanofibers increased with increasing diameters. The changes in extension of single CsgA fibers were found to be up to 17 fold that of the original length, indicating exceptional tensile properties. Our results provide new insights into the tensile properties of bacterial amyloid nanofibers and highlight the ultrahigh structural stability of theEscherichia colibiofilms.
机译：CSGA是Colibiofilms的主要蛋白质亚基，并在细菌粘附和侵袭中发挥关键作用。 CSGA蛋白可以自组装成淀粉样蛋白纳米纤维，其特征在于它们的分层结构，跨多个长度尺度，突出强度及其在恶劣环境下的结构稳健性。这里，磁性镊子用于研究纤维水平的CSGA蛋白的力谱。单个纳米纤维的两端直接使用先前报道的无标记方法在磁珠和玻璃滑块之间连接。我们表明，可以应用蠕虫状链模型以适应CSGA纳米纤维的典型力 - 延伸曲线并相应地估计机械性能。纳米纤维的弯曲刚度随着直径的增加而增加。发现单个CSGA纤维的延伸的变化达到原始长度的17倍，表示出色的拉伸性能。我们的研究结果为细菌淀粉样氧化物纳米纤维的拉伸性能提供了新的见解，并突出了Colibiofilms的超高结构稳定性。

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  • 来源
    《RSC Advances》 |2020年第37期|共7页
  • 作者

    Lv Jingqi; Li Yingfeng; Zhou Kai; Guo Pei; Liu Yang; Ding Ke; Li Ke; Zhong Chao; Xiao Botao;

  • 作者单位

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

    ShanghaiTech Univ Sch Phys Sci &

    Technol Mat &

    Phys Biol Div Shanghai 201210 Peoples R China;

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

    ShanghaiTech Univ Sch Phys Sci &

    Technol Mat &

    Phys Biol Div Shanghai 201210 Peoples R China;

    ShanghaiTech Univ Sch Phys Sci &

    Technol Mat &

    Phys Biol Div Shanghai 201210 Peoples R China;

    South China Univ Technol Sch Biol &

    Biol Engn Joint Int Res Lab Synthet Biol &

    Med Guangzhou 510006 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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