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首页> 外文期刊>RSC Advances >Enhanced ethanol production from sugarcane molasses by industrially engineered Saccharomyces cerevisiae via replacement of the PHO4 gene
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Enhanced ethanol production from sugarcane molasses by industrially engineered Saccharomyces cerevisiae via replacement of the PHO4 gene

机译：通过替代PHO4基因，通过工业化工程化酿酒酵母从甘蔗糖蜜产生增强乙醇生产

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摘要

Replacement of a novel candidate ethanol fermentation-associated regulatory gene, PHO4, from a fast-growing strain MC15, as determined through comparative genomics analysis among three yeast strains with significant differences in ethanol yield, is hypothesised to shorten the fermentation time and enhance ethanol production from sugarcane molasses. This study sought to test this hypothesis through a novel strategy involving the transfer of the PHO4 gene from a low ethanol-producing, yet fast-growing strain MC15 to a high ethanol-producing industrial strain MF01 through homologous recombination. The results indicated that PHO4 in the industrially engineered strain MF01-PHO4 displayed genomic stability with a mean maximum ethanol yield that rose to 114.71 g L-1, accounting for a 5.30% increase in ethanol yield and 12.5% decrease in fermentation time in comparison with that in the original strain MF01, which was the current highest ethanol-producing strain in SCM fermentation in the reported literature. These results serve to advance our current understanding of the association between improving ethanol yield and replacement of PHO4, while providing a feasible strategy for industrially engineered yeast strains to improve ethanol production efficiently.
机译：通过三种酵母菌株中的三种酵母菌株测定的快速增长的菌株MC15取代新的候选乙醇发酵相关调控基因，PHO4，通过三种酵母菌株，其假设缩短发酵时间并增强乙醇生产来自甘蔗糖蜜。本研究试图通过一种通过同源重组将PHO4基因从低乙醇产生，速度的菌株MC15转移到高乙醇产物产业菌株MFO1的新型策略来测试这一假设。结果表明，工业化学菌株MF01-PHO4中的PHO4呈现出基因组稳定性，其平均最大乙醇产率升至114.71g L-1，占乙醇产率增加的5.30％，发酵时间的12.5％降低相比在原始菌株MF01中，这是报告文献中的SCM发酵中的当前最高乙醇产生菌株。这些结果有助于推动我们目前对改善乙醇产量和更换PHO4之间的关联的理解，同时为工业设计酵母菌株提供可行的策略，以有效地改善乙醇生产。

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  • 来源
    《RSC Advances》 |2020年第4期|共10页
  • 作者

    Wu Renzhi; Chen Dong; Cao Shuwei; Lu Zhilong; Huang Jun; Lu Qi; Chen Ying; Chen Xiaoling; Guan Ni; Wei Yutuo; Huang Ribo;

  • 作者单位

    Guangxi Univ Coll Life Sci &

    Technol State Key Lab Conservat &

    Utilizat Subtrop Agrobi 100 Daxue Rd Nanning 530004 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Univ Coll Life Sci &

    Technol State Key Lab Conservat &

    Utilizat Subtrop Agrobi 100 Daxue Rd Nanning 530004 Guangxi Peoples R China;

    Guangxi Univ Coll Life Sci &

    Technol State Key Lab Conservat &

    Utilizat Subtrop Agrobi 100 Daxue Rd Nanning 530004 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Acad Sci Guangxi Key Lab Biorefinery State Key Lab Nonfood Biomass Enzyme Technol Natl Engn Res Ctr Nonfood Biorefinery 98 Daling Rd Nanning 530007 Guangxi Peoples R China;

    Guangxi Univ Coll Life Sci &

    Technol State Key Lab Conservat &

    Utilizat Subtrop Agrobi 100 Daxue Rd Nanning 530004 Guangxi Peoples R China;

    Guangxi Univ Coll Life Sci &

    Technol State Key Lab Conservat &

    Utilizat Subtrop Agrobi 100 Daxue Rd Nanning 530004 Guangxi Peoples R China;

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