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首页> 外文期刊>RSC Advances >An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters
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An aptasensor for the label-free detection of thrombin based on turn-on fluorescent DNA-templated Cu/Ag nanoclusters

机译：基于开启荧光DNA模板Cu / Ag纳米能器的凝血酶对凝血酶的无标记检测的Aptasensor

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摘要

A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs). Two DNA templates were designed as the functional structures consisting of the Cu/Ag NC-nucleation segment located at two termini or one terminus and the aptamer segment in the middle of a DNA template. Two darkish DNA-Cu/Ag NCs came close to each other when the aptamer combined with the target due to the conformational alteration of the aptamer structure, resulting in an increased fluorescence signal readout. Thrombin was sensitively determined as low as 1.6 nM in the range of 1.6-8.0 nM with a high selectivity. Finally, this sensor succeeded in detecting thrombin in a real fetal bovine serum.
机译：基于由目标识别的抗粉体构象的改变和由于两种黑暗的DNA模板铜/银纳米团簇（DNA-CU / AG NC）的接近度而构建高敏感的凝血酶AptaseSoR。 设计了两种DNA模板作为由位于末端或一个末端的Cu / Ag NC-成核段组成的功能结构，以及DNA模板中间的一个末端和Aptamer段。 当Aptamer由于适于结构构象结构而与目标相结合时，两种黑暗的DNA-Cu / Ag NCS彼此接近，导致荧光信号读出增加。 凝血酶在1.6-8.0nm的范围内敏感地低至1.6nm，具有高选择性。 最后，该传感器成功地检测真正胎牛血清中的凝血酶。

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  • 来源
    《RSC Advances》 |2020年第58期|共7页
  • 作者

    Zhang Baozhu; Wei Chunying;

  • 作者单位

    Jinzhong Univ Coll Chem &

    Chem Engn Yuci 030619 Peoples R China;

    Shanxi Univ Inst Mol Sci Minist Educ Key Lab Chem Biol &

    Mol Engn Taiyuan 030006 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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