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Photo-initiated rupture of azobenzene micelles to enable the spectroscopic analysis of antimicrobial peptide dynamics

机译：偶氮苯胶束的光引发破裂，使抗菌肽动力学的光谱分析

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Antimicrobial peptides (AMPs) show promise for the treatment of bacterial infections, but many have undesired hemolytic activities. The AMP MP1 not only has broad spectrum bactericidal activity, but has been shown to have antitumor activity. The interaction between AMPs and cellular membranes gives rise to a peptide's cell-specificity and activity. However, direct analysis of the biophysical interactions between peptides and membrane is complex, in part due to the nature of membrane environments as well as structural changes in the peptide that occurs upon binding to the membrane. In order to investigate the interplay between cell selectivity, activity, and secondary structural changes involved in antimicrobial peptide activity, we sought to implement photolizable membrane mimics to assess the types of information available from infrared spectroscopic measurements that follow from photoinitiated peptide dynamics. Azo-surfactants (APEG) form micelles containing a photolizable azobenzene core, which upon irradiation can induce membrane deformation resulting in breakdown of micelles. Spectroscopic analysis of membrane deformation may provide insights into the physical behavior associated with unfolding and dissociation of antimicrobial peptides within a membrane environment. Herein, we synthesized and characterized two new azo-surfactants, APEGTMGand APEGNEt2MeI. Furthermore, we demonstrate the viability of azosurfactants as membrane mimics by examining both the membrane binding and dissociation induced secondary structural changes of the antimicrobial peptide, MP1.
机译：抗菌肽（安培）用于细菌感染的治疗有希望，但许多有不需要的溶血活性。该AMP MP1不仅具有广谱的杀菌活性，但已经显示出具有抗肿瘤活性。 AMPS和细胞膜之间的相互作用产生了肽的细胞特异性和活性。然而，肽和膜之间的生物物理相互作用的直接分析是复杂的，部分原因是由于膜的环境以及在结合后的膜发生肽结构变化的性质。为了研究细胞选择性，活性，以及​​参与抗微生物肽的活性的二级结构的变化之间的相互影响，我们试图实现photolizable膜模拟物，以评估可从来自光引发肽动力学遵循红外光谱测量的类型的信息。含有photolizable偶氮苯核，其中在照射时能够诱导导致微胶粒的击穿膜变形偶氮系表面活性剂（APEG）形成胶束。膜变形的分光分析可以提供深入了解与膜环境中解折叠和抗微生物肽的解离相关联的物理行为。在此，我们合成并表征了两个新的偶氮系表面活性剂，APEG TMG 和APEG 净 2 加入MeI 。此外，我们通过检查两个膜结合并诱导抗微生物肽，MP1的二级结构变化的解离表明azosurfactants作为膜模拟物的生存力。

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