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首页> 外文期刊>RSC Advances >Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa
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Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa

机译：从Trapa Quadrispinosa茎的粗多糖的提取，初步表征和抗炎活性的优化

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摘要

A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM). At a water-to-material fixed ratio of 30 mL g(-1), the highest yield of 3.72 +/- 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 degrees C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%. Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature. The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis. In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-alpha, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner. These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
机译：使用加压辅助萃取（PAE）开发了一种新的方法，以有效地从植物茎中提取粗Trapa Quadrispinosa多糖（TQCPS），并且使用响应面法（RSM）优化提取过程。在水 - 材料固定比率为30ml g（-1），在32分钟的提取时间的最佳条件下获得3.72 +/- 0.13％的最高收率，提取温度为47℃，提取1.87 MPa的压力，这与预测值为3.683％。与传统的热水萃取（HWE）相比，PAE方法具有显着提高提取产率，提取时间短，提取温度低的进一步优点。通过UV-Vis，FT-IR和化学成分分析分析TQCP的初步特征。在随后的抗炎性研究中，当用TQCPS处理原料264.7小鼠巨噬细胞时，观察到令人满意的抗炎活性，并且TQCPS显着抑制了一氧化氮（NO），肿瘤坏死因子-α（TNF-α）的释放，和白细胞介素-6（IL-6）并同步抑制脂多糖（LPS）诱导的脂多糖（LPS）的诱导型一氧化氮合酶（INOS），TNF-α和IL-6 mRNA的表达水平以剂量依赖性方式。这些结果表明，PAE是一种可用于有效提取来自药用植物多糖的技术，并且TQCPS可以探索为医学中的潜在的抗炎剂。

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  • 来源
    《RSC Advances》 |2019年第39期|共11页
  • 作者

    Li Feng; Liu Xinhu; Yu Xiaofeng; Xu Xiuquan; Yang Huan;

  • 作者单位

    Jiangsu Univ Affiliated Hosp Zhenjiang 212001 Jiangsu Peoples R China;

    Taizhou Natl Med Hitech Dev Zone Taizhou 225300 Peoples R China;

    Jiangsu Univ Sch Pharm Zhenjiang 212013 Jiangsu Peoples R China;

    Jiangsu Univ Sch Pharm Zhenjiang 212013 Jiangsu Peoples R China;

    Jiangsu Univ Sch Pharm Zhenjiang 212013 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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