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Isolation and identification of an antioxidant collagen peptide from skipjack tuna (Katsuwonus pelamis) bone

机译：来自Skipjack金枪鱼（Katsuwonus pelamis）骨的抗氧化胶原肽的分离和鉴定

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To date, many researchers have developed active components that are derived from seafood processing for the purposes of healthcare. Here, an antioxidant collagen peptide was obtained from skipjack tuna (Katsuwonus pelamis) bone by using a combination of trypsin and chymotrypsin as the catalyst. The amino acid sequence of the peptide was identified as Ser-Ser-Gly-Pro-Pro-Val-Pro-Gly-Pro-Met-Gly-Pro-Met-Gly-Pro-Arg (SSGPPVPGPMGPMGPR) by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) analysis. We found that the as-prepared collagen peptide can efficiently scavenge DPPH radical (IC50 3.149 mM), superoxide anion radical (IC50 3.803 mM) and ABTS radical (IC50 9.489 mM). In addition, it has been found that the methionine (Met) residue in the collagen peptide could provide a precise active site during the scavenging of DPPH radicals by Fourier transform infrared spectroscopy (FTIR) analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. These results suggest that the peptide can find wide uses in the food, cosmetic and pharmaceutical industries.
机译：迄今为止，许多研究人员已经开发出用于医疗保健目的的海鲜处理的活性组件。这里，通过使用胰蛋白酶和胰凝乳蛋白酶的组合作为催化剂，从Skipjack金枪鱼（Katsuwonus pelamis）骨中获得抗氧化胶原蛋白。通过液相色谱 - 电喷雾电离将肽的氨基酸序列鉴定为Ser-Ser-Gly-pro-pro-pro-pro-pro-pro-pro-pro-pro-gly-pro-pro-pro-pro-pro-pro-pro-pro-pro-pro-pro-pro-pro-arg-pro-pro-pro-pro-arg-pro-pro-pro-arg（ssgppvpgpmgpmgpr）四极其飞行时间质谱（LC-ESI-QTOF-MS）分析。我们发现，制备的胶原蛋白肽可以有效地清除DPPH自由基（IC50 3.149mm），超氧化物阴离子（IC50 3.803mm）和ABTS自由基（IC50 9.489mm）。此外，已经发现胶原蛋白肽中的甲硫氨酸（Met）残基可通过傅里叶变换红外光谱（FTIR）分析和矩阵辅助激光解吸/电离时间来提供在DPPH自由基的清除期间的精确活性位点-Flight（MALDI-TOF）质谱分析。这些结果表明，肽可以在食品，化妆品和制药行业中找到广泛的用途。

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    《RSC Advances》 |2019年第46期|共10页
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  • 正文语种 eng
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