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Microwave plasma-assisted silicon nanoparticles: cytotoxic, molecular, and numerical responses against cancer cells

机译：微波等离子体辅助硅纳米粒子：细胞毒性，分子和对癌细胞的数值反应

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摘要

Silicon nanoparticles (SiNPs), which have a special place in material science due to their strong luminescent property and wide applicability in various physicochemical arenas, such as solar cells and LEDs, were synthesised by a microwave plasma-assisted process using an argon-silane mixture. Several characterization tools were applied to check the crystallinity (XRD) and morphological (FESEM, TEM, approximate to 20 +/- 2 nm size) and topographical (AFM, approximate to 20 nm) details of the NPs. The high-purity SiNPs were applied on myoblast cancer cells to investigate the reactivity of the NPs at different doses (200, 1000 and 2000 ng mL(-1)) for different incubation periods (24 h, 48 h & 72 h). The MTT assay was utilized to determine the percentage of viable and non-viable cells, while the cell organization was observed via microscopy and CLSM. Additionally, the molecular responses (RT-PCR), such as apoptosis, were analyzed in presence of caspase 3 and 7, and the results showed an upregulation with SiNPs. To validate the obtained data, analytical studies were also performed for the SiNPs via statistical analysis and the most reliable data values were evaluated and acceptable as per the ICH guidelines.
机译：通过使用氩硅烷混合物的微波等离子体辅助工艺合成了由于其强大的发光性质和各种物理化学竞技场中的硅纳米粒子（SINPS）具有特殊的物质科学中的特殊位置。使用氩硅烷混合物，通过微波等离子体辅助方法合成。应用了几种表征工具来检查结晶度（XRD）和形态学（FeSem，TEM，近似为20 +/-2nm尺寸）和地形（AFM，近似为20nm）细节。高纯度SINPS施用于肌细胞癌细胞，以研究不同剂量（200,1000和2000ng mL（-1））的NPS的反应性，用于不同孵育期（24小时，48小时72小时）。使用MTT测定来确定可行性和不活细胞的百分比，而通过显微镜和CLSM观察细胞组织。另外，在Caspase 3和7的存在下分析了分子响应（RT-PCR），例如凋亡，结果表明，结果表明了鼻窦的上调。为了验证所获得的数据，还通过统计分析对SINPS进行分析研究，并根据ICH指南评估和可接受的数据值。

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  • 来源
    《RSC Advances》 |2019年第23期|共12页
  • 作者

    Wahab Rizwan; Khan Farheen; Gupta Anoop; Wiggers Hartmut; Saquib Quaiser; Faisal Mohammad; Ansari Sabiha Mahmood;

  • 作者单位

    King Saud Univ Coll Sci Zool Dept POB 2455 Riyadh 11451 Saudi Arabia;

    Taibah Univ Fac Sci Chem Dept Yanbu Saudi Arabia;

    Univ Duisburg Essen Inst Combust &

    Gas Dynam Duisburg Germany;

    Univ Duisburg Essen Inst Combust &

    Gas Dynam Duisburg Germany;

    King Saud Univ Coll Sci Zool Dept POB 2455 Riyadh 11451 Saudi Arabia;

    King Saud Univ Coll Sci Dept Bot &

    Microbiol POB 2455 Riyadh 11451 Saudi Arabia;

    King Saud Univ Coll Sci Dept Bot &

    Microbiol POB 2455 Riyadh 11451 Saudi Arabia;

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  • 正文语种 eng
  • 中图分类 化学;
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