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首页> 外文期刊>RSC Advances >Photo-patterned oxygen sensing films based on Pt porphyrin for controlling cell growth and studying metabolism
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Photo-patterned oxygen sensing films based on Pt porphyrin for controlling cell growth and studying metabolism

机译：基于Pt卟啉的光学图案氧传感薄膜控制细胞生长和研究代谢的研究

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摘要

A new type of biocompatible and photo-polymerizable hydrogel with oxygen sensors for microengineering was developed. Herein, a red emitter as an oxygen probe which was chemically immobilized in a poly(2-hydroxyethyl methacrylate)-co-polyacrylamide-based matrix was expected to monitor cell metabolism. A few micropatterned films with gratings (5, 7, 10, 20, and 50 m in width, respectively and with 1.2 m in height) were designed and fabricated by photo-lithography using these hydrogels. SEM and AFM were used to validate these films to attain their lateral width and vertical depth. The oxygen responses of these films were characterized. Results showed that patterned films exhibited higher sensitivity than the non-patterned films. The films' construction can also have some influence on cell alignment and elongation. This phenomenon was evaluated by culturing human cervical cancer cells (HeLa cells) and mouse embryo fibroblasts (3T3-L1), on the film surfaces with different construction. Linear correlation between cell elongation and the logarithm of grating width was observed. Real-time monitoring of oxygen consumption of HeLa cells in cell culture medium was achieved. This study is expected to have potential to be applied in micro-structured design and to help understanding metabolism.
机译：开发了一种新型的生物相容性和可光聚合水凝胶，其具有用于微版中的微生能的氧传感器。这里，预期在聚（2-羟乙基甲基丙烯酸酯）-CO-聚丙烯酰胺基基质中化学固定的氧气探针的红色发射极将监测细胞代谢。使用这些水凝胶的光光刻设计和制造了一些具有光栅（5,7,10,20和50μm的微型薄膜（5,7,10,20和50μm），使用这些水凝胶制造和制造。 SEM和AFM用于验证这些薄膜以获得横向宽度和垂直深度。表征了这些薄膜的氧气反应。结果表明，图案化薄膜表现出比非图案薄膜更高的敏感性。电影的结构也可以对细胞对准和伸长率产生一些影响。通过在具有不同结构的膜表面上培养人宫颈癌细胞（Hela细胞）和小鼠胚胎成纤维细胞（3T3-L1）来评估这种现象。观察到电池伸长和光栅宽度对数之间的线性相关性。实现了细胞培养基中HeLa细胞氧气消耗的实时监测。预计本研究将有可能以微结构化设计应用并帮助理解新陈代谢。

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  • 来源
    《RSC Advances》 |2019年第2期|共7页
  • 作者

    Zeng Fei; Fan Zengju; Wu Shanshan; Cheng Xing; Tian Yanqing;

  • 作者单位

    Southern Univ Sci &

    Technol Dept Mat Sci &

    Engn 1088 Xueyuan Blvd Shenzhen 518055 Guangdong Peoples R China;

    Southern Univ Sci &

    Technol Dept Mat Sci &

    Engn 1088 Xueyuan Blvd Shenzhen 518055 Guangdong Peoples R China;

    Guangdong Ind Polytech 152 Xingang West Rd Guangzhou 510300 Guangdong Peoples R China;

    Southern Univ Sci &

    Technol Dept Mat Sci &

    Engn 1088 Xueyuan Blvd Shenzhen 518055 Guangdong Peoples R China;

    Southern Univ Sci &

    Technol Dept Mat Sci &

    Engn 1088 Xueyuan Blvd Shenzhen 518055 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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