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A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide

机译：基于RNA适体与石墨烯氧化物相互作用的茶碱荧光传感平台

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摘要

RNA, with a structure similar to DNA, should exhibit similar behaviors when it interacts with graphene. In this work, we designed a sensing platform of theophylline based on the interaction of an RNA aptamer with graphene oxide (GO) using the fluorescence as a sensing signal. Firstly, quantum dots (QDs) were modified with the selected ssRNA that can be used as an aptamer to recognize the theophylline. The fluorescence of QDs will be quenched in the presence of GO due to the noncovalent assembly between ssRNA aptamer and GO, leading to fluorescence resonance energy transfer (FRET) from QDs to GO, fluorescence "turn-off". Then, in the presence of theophylline, the ssRNA aptamer recognizes theophylline to form a dsRNA-theophylline complex. The weak affinity between the complex and GO makes QDs move away from the GO surface, leading to the fluorescence recovery of QDs, fluorescence "turn-on". Because of the high fluorescence quenching efficiency, unique structure of GO and specificity of the RNA aptamer, the proposed sensing platform exhibits high sensitivity and excellent selectivity for the determination of theophylline. The excellent performance of the sensor based on GO provides new opportunities for sensitive and selective detection of biorecognition events.
机译：具有类似于DNA的结构的RNA应在与石墨烯相互作用时表现出类似的行为。在这项工作中，我们设计了基于RNA适体与石墨烯氧化物（GO）的相互作用的茶碱的感测平台，所述荧光作为传感信号。首先，用所选择的SSRNA修饰量子点（QDS），所述SSRNA可用作识别茶碱的适体。由于SSRNA适体之间的非共价组装，QDS的荧光将在发生的情况下淬火，导致QDS的荧光共振能量转移（FRET）进行荧光“关断”。然后，在茶碱存在下，SSRNA适体识别茶碱形成DsRNA-茶茶碱基。复杂和转移之间的弱亲和力使QDS远离去表面，导致QD的荧光回收，荧光“开启”。由于荧光猝灭效率高，GO的独特结构和RNA适体的特异性，所提出的传感平台具有高灵敏度和优异的选择性，用于确定茶碱。基于Go的传感器的出色性能为生物识别事件的敏感和选择性检测提供了新的机会。

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  • 来源
    《RSC Advances》 |2019年第34期|共6页
  • 作者

    Wu Jian-Feng; Gao Xin; Ge Ling; Zhao Guang-Chao; Wang Guang-Feng;

  • 作者单位

    Anhui Normal Univ Coll Environm Sci &

    Engn Anhui Prov Engn Lab Water &

    Soil Pollut Control &

    Wuhu Peoples R China;

    Anhui Normal Univ Coll Environm Sci &

    Engn Anhui Prov Engn Lab Water &

    Soil Pollut Control &

    Wuhu Peoples R China;

    Anhui Normal Univ Coll Environm Sci &

    Engn Anhui Prov Engn Lab Water &

    Soil Pollut Control &

    Wuhu Peoples R China;

    Anhui Normal Univ Coll Environm Sci &

    Engn Anhui Prov Engn Lab Water &

    Soil Pollut Control &

    Wuhu Peoples R China;

    Anhui Normal Univ Coll Environm Sci &

    Engn Anhui Prov Engn Lab Water &

    Soil Pollut Control &

    Wuhu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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