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首页> 外文期刊>RSC Advances >Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase from Sinorhizobium sp.
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Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase from Sinorhizobium sp.

机译：SINORHIZOBIUM SP的新型D-Tagatose 3-eBimerase的生物化学表征及生物催化应用。

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摘要

Sinorhizobium sp. D-tagatose 3-epimerase (sDTE) catalyzes the conversion of D-tagatose to D-sorbose. It also recognizes D-fructose as a substrate for D-allulose production. The optimal temperature and pH of the purified sDTE was 50 degrees C and 8.0, respectively. Based on the sDTE homologous model, Glu154, Asp187, Gln213, and Glu248, form a hydrogen bond network with the active-site Mn2+ and constitute the catalytic tetrad. The amino acid residues around O-1, -2, and -3 atoms of the substrates (D-tagatose/D-fructose) are strictly conserved and thus likely regulate the catalytic reaction. However, the residues at O-4, -5, and -6, being responsible for the substrate-binding, are different. In particular, Arg65 and Met9 were found to form a unique interaction with O-4 of D-fructose and D-tagatose. The whole cells with recombinant sDTE showed a higher bioconversion rate of 42.5% in a fed-batch bioconversion using D-fructose as a substrate, corresponding to a production of 476 g L-1 D-allulose. These results suggest that sDTE is a potential industrial biocatalyst for the production of D-allulose in fed-batch mode.
机译：Sinorhizobium sp。 D-Tagatose 3-eBimerase（SDTE）催化D-Tagatose至D-山梨糖的转化。它还识别D-果糖作为D-甲型制备的基材。纯化的SDTE的最佳温度和pH分别为50℃和8.0。基于SDTE同源模型，GLU154，ASP187，GLN213和GLU248，形成具有有源部位MN2 +的氢键网络，并构成催化Tetrad。邻接O-1，-2和-3原子的氨基酸残基严格保守，因此可能调节催化反应。然而，O-4，-5和-6的残基对底物结合负责是不同的。特别地，发现Arg65和Met9形成与D-果糖和D-塔肽的O-4的独特相互作用。具有重组SDTE的整个细胞使用D-果糖作为基材的FED-批量生物转化率较高的生物转化率为42.5％，对应于476g L-1 D-甲壳的产生。这些结果表明，SDTE是潜在的工业生物催化剂，用于在氟批模式下生产D-甲型。

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  • 来源
    《RSC Advances》 |2019年第6期|共9页
  • 作者

    Zhu Zhangliang; Li Chao; Liu Xin; Gao Dengke; Wang Xueyu; Tanokura Masaru; Qin Hui-Min; Lu Fuping;

  • 作者单位

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

    Tianjin Univ Sci &

    Technol Key Lab Ind Fermentat Microbiol Tianjin Key Lab Ind Microbiol Minist Educ Coll Biotechnol Natl Engn Lab Ind Enz Tianjin 300457 Peoples R China;

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