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Exploring the water/oil/water interface of phospholipid stabilized double emulsions by micro-focusing synchrotron SAXS

机译：通过微聚焦同步凝固套管探索磷脂稳定的双乳液的水/油/水界面

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Surfactant stabilized water/oil/water (w/o/w) double emulsions have received much attention in the last years motivated by their wide applications. Among double emulsions, those stabilized by phospholipids present special interest for their imitation of artificial cells, allowing the study of the effect of confining chemical reactions in biomimetic environments. Upon evaporation of the oil shell, phospholipid stabilized double emulsions can also serve as templates for giant vesicles. In this context, general assumptions have been made on the self-assembly and structural organization/arrangement of amphiphilic molecules, at the aqueous/oil liquid interface. However, to the best of our knowledge, no detailed evidence of the interfacial structuring have been reported. In this paper, w/o/w double emulsions formulated using the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and a mixture of chloroform and cyclohexane as the oil phase were produced using a microfluidic device. To obtain information on the phospholipid arrangement, the w/o/w interface was investigated by spatially resolved micro-focusing SAXS. We observed that (i) the basic units forming both the w/o and o/w interfaces were oil-swollen DMPC bilayers, arranged into a substantially disordered shell of similar to 45 mu m thickness surrounding the internal oil phase; (ii) the evaporation process was slow, i.e. in the order of one hour at 50 degrees C and (iii) oil evaporation led to a shrinkage of the interfacial shell, but not to an increase of the ordering of the lipid bilayers. Interestingly, no stacked DMPC bilayers were observed during the evaporation process, as shown by the absence of Bragg's peaks in the SAXS intensity profiles.
机译：表面活性剂稳定的水/油/水（W / O / W）双乳液在其广泛应用程序的最后一年中受到了很多关注。在双乳液中，通过磷脂稳定的那些对其仿制人工细胞的特殊兴趣，允许研究在仿生环境中限制化学反应的影响。在蒸发油壳后，磷脂稳定的双乳液也可用作巨囊囊泡的模板。在这种情况下，在含水/油液界面处，已经对自组装和结构组织/排列进行了一般假设。然而，据我们所知，没有报告界面结构的详细证据。在本文中，使用微流体装置制备使用磷脂1,2-二巯基-Sn-甘油-3-普华啉（DMPC）和氯仿和环己烷的混合物配制的W / O / W双乳液。为了获得有关磷脂布置的信息，通过空间分辨的微聚焦淋巴研究了W / O / W界面。我们观察到（i）形成W / O和O / W界面的基本单元是油溶胀的DMPC双层，布置成与内部油相周围的45μm厚度相似的基本无序的壳; （ii）蒸发过程缓慢，即在50摄氏度为1小时的顺序，（iii）漏流导致界面壳的收缩，但不增加脂质双层的排序。有趣的是，在蒸发过程中没有观察到堆叠的DMPC双层，如萨克斯强度谱中的缺失的布拉格峰的情况所示。

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    《RSC Advances》 |2019年第57期|共7页
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