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Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues

机译:用于二次检测硝基呋喃代谢物残留的超敏感条带

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摘要

In this biosensor system, metabolite residues were derived by using a previous B-CBA synthesis method to label a biotin moiety for enrichment by streptavidin coated magnetic beads. Antibodies specific for derivatives were conjugated with carboxyl-modified barcode DNAs which were used as templates for strand displacement amplification (SDA). The assay can detect trace levels of 7.20 ppt of SEM, 11.58 ppt of AHD, 7.24 ppt of AOZ and 2.31 ppt of AMOZ, respectively.
机译:在这种生物传感器系统中,通过使用先前的B-CBA合成方法来衍生代谢物残留方法,以通过链霉抗生物素蛋白涂覆的磁珠来标记生物素部分以富集。 与羧基改性的条形码DNA缀合特异性衍生物的抗体,其用作链位移扩增(SDA)的模板。 测定可以分别检测AOZ和2.31 PPT的SEM,11.58 PPT的SEM,11.58 ppt的痕量水平。

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  • 来源
    《RSC Advances》 |2019年第5期|共4页
  • 作者单位

    Qingdao Agr Univ Coll Food Sci &

    Engn 700 Changcheng Rd Qingdao 266179 Peoples R China;

    Guangzhou Med Univ Affiliated Hosp 5 78 Hengzhigang Rd Guangzhou 510095 Guangdong Peoples R China;

    Qingdao Univ Affiliated Hosp Dept Ophthalmol 1677 Wutaishan Rd Qingdao Peoples R China;

    Guangzhou Med Univ Affiliated Hosp 5 78 Hengzhigang Rd Guangzhou 510095 Guangdong Peoples R China;

    Guangzhou Med Univ Affiliated Hosp 5 78 Hengzhigang Rd Guangzhou 510095 Guangdong Peoples R China;

    Qingdao Univ State Key Lab Biochem Engn Inst Proc Engn Qingdao 266003 Peoples R China;

    Guangzhou Med Univ Affiliated Hosp 5 78 Hengzhigang Rd Guangzhou 510095 Guangdong Peoples R China;

    Qingdao Agr Univ Coll Food Sci &

    Engn 700 Changcheng Rd Qingdao 266179 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

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