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ACE-inhibitory and antioxidant peptides from coconut cake albumin hydrolysates: purification, identification and synthesis

机译：来自椰子蛋白聚糖水解产物的ACE-抑制和抗氧化剂肽：纯化，鉴定和合成

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摘要

Coconut cake albumin was hydrolysed by sequential digestion with alcalase, flavourzyme, pepsin and trypsin to purify bioactive peptides with ACE-inhibitory and antioxidant activities. Following fractionation with sequential ultrafiltration, Sephadex gel chromatography and RP-HPLC, three novel peptides KAQYPYV, KIIIYN and KILIYG were identified. KAQYPYV, KIIIYN and KILIYG provided an IC50 value of 37.06 mM, 58.72 mM and 53.31 mM on ACE-inhibitory activity, respectively. For hydroxyl radical scavenging activity, KAQYPYV, KIIIYN and KILIYG demonstrated an IC50 value of 70.84 mM, 77.62 mM and 95.23 mM, respectively. All the three peptides exhibited a mixed modality of noncompetitive and uncompetitive inhibition on ACE and KAQYPYV showed good stability against gastrointestinal enzymes digestion. Moreover, these peptides could effectively lower intracellular endothelin(-1) content without significant cytotoxicity, and protected vascular endothelial cells from reactive oxygen species mediated damage. Furthermore, KAQYPYV, KIIIYN and KILIYG also demonstrated high ion chelating ability (62.13%-4.21%, 64.66%-5.51% and 69.82%-7.24% at 0.1 mg mL(-1), respectively) and considerable superoxide radical scavenging activity (39.30%-2.72%, 46.79%(-1).70% and 51.16%-3.23% at 1.0 mg mL(-1), respectively). These results indicate that coconut cake albumin is a potential source of bioactive peptides possessing ACE-inhibitory and antioxidant activities.
机译：通过用alcalase，flavourzyme，胃蛋白酶和胰蛋白酶顺序消化用肝脏蛋白蛋白酶用椰子消化水解，以用ACE抑制和抗氧化活性净化生物活性肽。在用序贯超滤的分馏后，鉴定了三种新肽Kaqypyv，三种新肽Kaqypyv，Kiiiyn和Kiliyg。 KaqyPyv，Kiiiyn和Kiliyg分别为ACE抑制活性提供了37.06毫米，58.72毫米和53.31毫米的IC50值。对于羟基自由基清除活性，KaqyPyv，Kiiiyn和Kiliyg分别显示了IC50值，分别为70.84毫米，77.62毫米和95.23mm。所有三种肽在ACE和Kaqypyv上表现出非竞争性和非竞争性抑制的混合模态，并且Kaqypyv对胃肠道消化的稳定性良好。此外，这些肽可以有效地降低细胞内内皮素（-1）含量，而无需显着细胞毒性，并且受到反应性氧物种的保护血管内皮细胞介导的损伤。此外，KaqyPyv，Kiiiyn和Kiliyg还分别证明了高离子螯合能力（62.13％-4.21％，64.66％-5.51％和69.82％-7.24％，分别为0.1mg ml（-1））和相当大的超氧化物自由基清除活性（39.30 ％-2.72％，46.79％（ - 1）.70％和51.16％-3.23％，分别为1.0mg ml（-1））。这些结果表明，椰子蛋白蛋白是具有ACE抑制和抗氧化活性的生物活性肽的潜在来源。

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  • 来源
    《RSC Advances》 |2019年第11期|共12页
  • 作者

    Zheng Yajun; Li Yan; Li Guifeng;

  • 作者单位

    Shanxi Normal Univ Coll Food Sci Linfen 041004 Peoples R China;

    Shanxi Normal Univ Coll Food Sci Linfen 041004 Peoples R China;

    Shanxi Normal Univ Coll Food Sci Linfen 041004 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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