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首页> 外文期刊>RSC Advances >Enhancement of fluorescent resonant energy transfer and the antenna effect in DNA structures with multiple fluorescent dyes
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Enhancement of fluorescent resonant energy transfer and the antenna effect in DNA structures with multiple fluorescent dyes

机译：具有多种荧光染料的DNA结构中荧光谐振能量转移和天线效应的增强

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摘要

This study examines the use of surfactants and metal cations for the enhancement of long range fluorescent resonant energy transfer (FRET) and the antenna effect in double-stranded (ds) DNA structures formed by hybridization of 21mer oligonucleotides with three fluorescent TAMRA donor dyes and complementary 21mer oligonucleotides with one fluorescent Texas Red acceptor dye. In FRET ds-DNA structures, hydrophobic interactions between the fluorescent dyes in close proximity produces dimerization and quenching which reduces fluorescent emissions. For the neutral surfactant Triton X-100, dimerization and emission quenching in the FRET ds-DNA structures remain unaffected. The cationic surfactant CTAB (>100 mu M), which neutralizes the negatively charged ds-DNA backbone reduces TAMRA dye dimerization and emission quenching, and improves the Texas Red quantum yield, FRET efficiency and the antenna effect. While the negatively charged SDS surfactant does not reduce dimerization and emission quenching, addition of sodium cations (Na+) and magnesium cations (Mg2+) lead to a significant reduction in dimerization and emission quenching, and produce higher FRET efficiency and enhanced antenna effect. This study provides a viable strategy for using combinations of surfactants and cations to reduce fluorescent dye and other quenching mechanisms and improve the overall long distance FRET efficiency and the antenna effect in ds-DNA structures.
机译：本研究检测使用表面活性剂和金属阳离子的使用，用于增强远程荧光谐振能量转移（FRET）和通过用三种荧光TAMRA供体染料和互补的21mer寡核苷酸的双链（DS）DNA结构中的天线效应。 21毫莫核苷酸与一个荧光德克萨斯红色受体染料。在FRET DS-DNA结构中，荧光染料之间的疏水相互作用在紧密接近产生的二聚化和淬火，从而减少荧光排放。对于中性表面活性剂Triton X-100，FRET DS-DNA结构中的二聚和发射猝灭仍未受到影响。中和带负电的DS-DNA主链的阳离子表面活性剂CTAB（>100μm）降低了Tamra染料二聚化和排放猝灭，并提高了德克萨斯红量子产率，FRET效率和天线效应。虽然带负电的SDS表面活性剂不减少二聚化和排放猝灭，但加入阳离子（Na +）和镁阳离子（Mg2 +）导致二聚化和排放猝灭的显着降低，并产生更高的FRET效率和增强的天线效应。本研究提供了使用表面活性剂和阳离子组合的可行策略，以减少荧光染料和其他猝灭机构，并提高DS-DNA结构中的整体长距离效率和天线效应。

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  • 来源
    《RSC Advances》 |2017年第24期|共8页
  • 作者

    Oh Taeseok; Kim Sejung; Choi Jae-Young; Chang Haeun; Heller Michael J.;

  • 作者单位

    Univ Calif San Diego Mat Sci &

    Engn Program 9500 Gilman Dr San Diego CA 92093 USA;

    Univ Calif San Diego Dept NanoEngn 9500 Gilman Dr San Diego CA 92093 USA;

    Univ Calif San Diego Dept NanoEngn 9500 Gilman Dr San Diego CA 92093 USA;

    Univ Calif San Diego Dept NanoEngn 9500 Gilman Dr San Diego CA 92093 USA;

    Univ Calif San Diego Dept NanoEngn 9500 Gilman Dr San Diego CA 92093 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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