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首页> 外文期刊>RSC Advances >In vitro study of novel microparticle based silk fibroin scaffold with osteoblast-like cells for load-bearing osteo-regenerative applications
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In vitro study of novel microparticle based silk fibroin scaffold with osteoblast-like cells for load-bearing osteo-regenerative applications

机译：具有骨细胞样细胞的新微粒丝素蛋白支架的体外研究，用于携带载有骨质细胞的骨质细胞再生应用

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摘要

Silk Fibroin (SF) is today considered to be one of the most favorable materials for bone tissue engineering. We have prepared novel SF microparticle based 3D scaffolds, with appropriate pore size, pore interconnectivity and porosity, excellent mechanical properties and tunable bioresorption, while retaining the inherent biocompatibility of SF. These properties make them ideal candidates for osteoregenerative applications. Here, we report the in vitro cell viability, cell adhesion and proliferation with osetoblastic differentiation of MG 63 osteoblast-like cell line on these scaffolds. In addition, we have also modified the surface of these scaffolds using collagen type I and chitosan biopolymers. Our results show that although the SF scaffold does support in vitro cell attachment, proliferation and differentiation, this performance can be further enhanced using the surface coating approach. Also, the ALP activity and bone mineralization was found to be particularly superior in the chitosan modified scaffolds.
机译：丝素蛋白（SF）今天被认为是骨组织工程最有利的材料之一。我们制备了新型SF微粒的3D支架，具有适当的孔径，孔隙互连和孔隙率，优异的机械性能和可调谐生物吸收，同时保持SF的固有生物相容性。这些属性使其成为骨骼根系应用的理想候选者。在此，我们在这些支架上报告了在这些支架上的醚溶细胞的体外细胞活力，细胞粘附性和增殖与Mg 63成骨细胞样细胞系。此外，我们还使用胶原蛋白I和壳聚糖生物聚合物来修饰这些支架的表面。我们的研究结果表明，尽管SF支架确实支持体外细胞附着，增殖和分化，但可以使用表面涂层方法进一步增强这种性能。此外，发现ALP活性和骨矿化在壳聚糖改性支架中特别优越。

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  • 来源
    《RSC Advances》 |2017年第43期|共8页
  • 作者

    Parekh Nimisha; Hushye Chandni; Warunkar Saniya; Sen Gupta Sayam; Nisal Anuya;

  • 作者单位

    Natl Chem Lab Polymer Sci &

    Engn Dept HomiBhabha Rd Pune 411008 Maharashtra India;

    Poona Coll Dept Zool Pune 411001 Maharashtra India;

    Poona Coll Dept Zool Pune 411001 Maharashtra India;

    Indian Inst Sci &

    Educ Res Dept Chem Sci Kolkata 741246 India;

    Natl Chem Lab Polymer Sci &

    Engn Dept HomiBhabha Rd Pune 411008 Maharashtra India;

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  • 正文语种 eng
  • 中图分类 化学;
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