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首页> 外文期刊>RSC Advances >Individual and combined use of ginsenoside F2 and cyanidin-3-O-glucoside attenuates H2O2-induced apoptosis in HEK-293 cells via the NF-kappa B pathway
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Individual and combined use of ginsenoside F2 and cyanidin-3-O-glucoside attenuates H2O2-induced apoptosis in HEK-293 cells via the NF-kappa B pathway

机译：人参皂苷F2和Cyanidin-3-O-葡糖苷的个体和结合使用通过NF-Kappa B途径衰减HEK-293细胞中的H2O2诱导的细胞凋亡

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摘要

Ginsenosides have been regarded as the major active components of ginseng. Cyanidin-3-O-glucoside (C3G) is one of the best-known and investigated anthocyanins. Individual and combined protective effects of ginsenoside F2 and C3G against H2O2-induced apoptosis and the underlying mechanisms were explored in HEK-293 cells. The protein expression of cleaved caspase-3, Bcl-2, Bax, and cleaved PARP, and the levels of caspases-6, caspases-9, and lactate dehydrogenase (LDH), and mitochondria membrane potential (MMP) were measured as the apoptosis associated markers. The phosphorylation of nuclear factor kappa B (NF-kB) p65 was assessed by western blotting and qRT-PCR. When HEK-293 cells were stimulated with H2O2, individual F2 and C3G pre-incubation increased MMP, suppressed the leakage of LDH, significantly decreased the activities of caspases-6 and caspases-9, down-regulated the protein expression of cleaved caspase-3, Bax, and cleaved PARP, up-regulated the expression of Bcl-2, and down-regulated the protein and mRNA expressions of NF-kB p65. The synergistic effects were present in the combination F2 and C3G. The underlying mechanism of F2 and C3G in anti-apoptosis was in part relevant to the regulation of the mitochondria-mediated cell apoptotic pathway and the NF-kB pathway. The co-administration of F2 and C3G exhibited possible synergistic effects on the inhibition of H2O2-induced apoptosis.
机译：人参皂苷被认为是人参的主要活性成分。 Cyanidin-3-O-葡糖苷（C3G）是最可知和研究的花青素之一。在HEK-293细胞中探讨了人参皂苷F2和C3G对H 2 O 2诱导的细胞凋亡的个体和综合保护作用。作为凋亡，测定了切割的Caspase-3，Bcl-2，Bax和切割PARP的蛋白质表达，以及半胱天冬酶-6，木糖-9和乳酸脱氢酶（LDH）和线粒体膜电位（MMP）的水平相关标记。通过Western印迹和QRT-PCR评估核因子Kappa B（NF-KB）P65的磷酸化。当用H 2 O 2刺激HEK-293细胞时，单独的F2和C3G预孵育增加MMP，抑制了LDH的泄漏，显着降低了Caspases-6和Caspases-9的活性，下调了切割的Caspase-3的蛋白质表达，Bax和Cleaved PARP，上调Bcl-2的表达，下调NF-Kb P65的蛋白质​​和mRNA表达。协同效应存在于组合F2和C3G中。 F2和C3G在抗细胞凋亡中的潜在机制部分与线粒体介导的细胞凋亡途径和NF-KB途径的调节有关。 F2和C3G的共同施用对抑制H2O2诱导的细胞凋亡表现出可能的协同作用。

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  • 来源
    《RSC Advances》 |2017年第66期|共10页
  • 作者

    Liu Di; Wang Ying; Ma Shuang; Sun Hongyu; Shi Wenyan; Feng Xianmin;

  • 作者单位

    Jilin Med Univ Dept Pathogen Biol Jilin 132013 Jilin Peoples R China;

    Jilin Univ Coll Food Sci &

    Engn Changchun 130062 Jilin Peoples R China;

    Jilin Univ Coll Food Sci &

    Engn Changchun 130062 Jilin Peoples R China;

    Jilin Med Univ Dept Pathogen Biol Jilin 132013 Jilin Peoples R China;

    Jilin Med Univ Dept Pathogen Biol Jilin 132013 Jilin Peoples R China;

    Jilin Med Univ Dept Pathogen Biol Jilin 132013 Jilin Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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