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首页> 外文期刊>RSC Advances >Individual and combined antioxidant effects of ginsenoside F2 and cyanidin-3-O-glucoside in human embryonic kidney 293 cells
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Individual and combined antioxidant effects of ginsenoside F2 and cyanidin-3-O-glucoside in human embryonic kidney 293 cells

机译：人参皂苷F2和Cyanidin-3-O-葡糖苷在人胚胎肾293细胞中的个体和结合抗氧化作用

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Ginsenosides and anthocyanins have already been reported to exhibit protective effects on intracellular defense against oxidative stress. Nuclear factor erythroid-derived 2 (NF-E2)-like 2 (Nrf2) plays a key role in signal pathways to counteract oxidative stress and controls expression of antioxidant enzymes. The present study is aimed at investigating the individual and combined antioxidant effects of ginsenoside F2 (F2) and cyanidin-3- O -glucoside (C3G) against H _(2) O _(2) -induced oxidative stress in Human Embryonic Kidney (HEK-293) cells. Cell viability, MDA level and activities of antioxidant enzymes were measured using corresponding assay kits. A DCFH-DA fluorescent probe assay was used to measure the level of intracellular reactive oxygen species (ROS). Quantitative real-time PCR and Western blotting were used to detect the expression of Nrf2 and Kelch-like ECH associated protein 1 (Keap1). The results showed that F2 and C3G significantly inhibited the generation of MDA and intracellular ROS, and increased the activities of antioxidant enzymes. Furthermore, F2 and C3G significantly enhanced the protein and mRNA expressions of Nrf2 and reduced the expressions of Keap1 in a concentration-dependent manner. On the basis of the stronger individual effects, synergistic effects were present in the combinations of F2 and C3G. In conclusion, these results demonstrated that F2 and C3G can protect HEK-293 cells against H _(2) O _(2) -induced oxidative stress and possibly act synergistically through reducing intracellular ROS, as well as activating the Nrf2/Keap1 signaling pathway and increasing antioxidant enzyme levels.
机译：已经报道了人参皂苷和花青素对氧化胁迫的细胞内防御表现出存在的保护作用。核因子红细胞衍生的2（NF-E2） - 样2（NRF2）在信号途径中起关键作用，以抵消氧化应激并控制抗氧化酶的表达。本研究旨在研究人参皂苷F2（F2）和Cyanidin-3-O-葡萄糖（C3G）对H _（2）O _（2） - 诱导人胚胎肾（1）的氧化胁迫（）的单独和组合抗氧化物（C3G）（ HEK-293）细胞。使用相应的测定试剂盒测量细胞活力，MDA水平和抗氧化酶的活性。使用DCFH-DA荧光探针测定测量细胞内反应性氧（ROS）的水平。定量实时PCR和Western印迹用于检测NRF2和keelch样ech相关蛋白1（Keap1）的表达。结果表明，F2和C3G显着抑制了MDA和细胞内RO的产生，并增加了抗氧化酶的活性。此外，F2和C3G显着增强了NRF2的蛋白质和mRNA表达，并以浓度依赖性方式减少了Keap1的表达。在较强的个体效果的基础上，在F2和C3G的组合中存在协同效应。总之，这些结果证明F2和C3G可以保护HEK-293细胞对H _（2）o _（2）诱导致氧化应激，并且可能通过减少细胞内ROS协同作用，以及激活NRF2 / Keap1信号通路增加抗氧化酶水平。

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