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One-step trapping of droplets and surface functionalization of sensors using gold-patterned structures for multiplexing in biochips

机译：使用金色图案结构在Biochips中复用传感器的一步诱捕和表面功能化

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Multiplexing in Point-of-Care (POC) diagnostics is crucial for a comprehensive readout of multifactorial diseases such as cancer or fast-acting complications such as heart attacks. In multiplexed POC (MPOC) testing, groups of sensors are functionalized with different biological probes, enabling the simultaneous capture and detection of various analytes. Current literature demonstrates MPOC systems using microfluidics, capable of compartmentalizing only a few sensing regions. In contrast, miniaturized robotic spotting (microspotting) theoretically enables the independent functionalization of every sensor in a microarray. However, its use is still challenging in biosensors which require the combination of different materials at the surface because microspotted droplets tend to move to areas of higher surface energy, thus affecting the spotting precision. To counteract this phenomenon, we have combined shapes of gold-patterned films for the functionalization of magnetoresistive (MR) sensors and simultaneous trapping of droplets over the sensing regions, previously passivated with silicon nitride (Si3N4). Due to the higher hydrophobicity of gold when compared to Si3N4, the droplets remain immobilized inside the gold frames and functionalization of the sensors is accurately achieved. With this strategy, we demonstrate the microspotting of different DNA probes and antibodies and the specific hybridization and binding of complementary DNA targets and proteins, attached to magnetic beads. This combined trapping and functionalization system shows promise in the implementation of MR-based systems in multiplexing applications. This work can also be of interest to the wider community working in the development of other surface-based assays, also developed through microfabrication techniques, as the same trapping methodology is conceivable for adaption to other biochip formats.
机译：在护理点（POC）诊断中复用对于综合读数的多重读出诸如癌症或快速作用并发症等心脏病发作的疾病至关重要。在多路复用的POC（MPOC）测试中，传感器组用不同的生物探针官能化，能够同时捕获和检测各种分析物。目前的文献演示了使用微流体的MPOC系统，能够仅包括几个传感区域。相反，小型化的机器人斑点（微型疗法）理论上使得能够在微阵列中独立于每个传感器的独立功能化。然而，它的使用仍然在生物传感器中仍然具有挑战性，这在表面处需要不同材料的组合，因为微杆液滴倾向于移动到更高的表面能的区域，从而影响斑点精度。为了抵消这种现象，我们具有用于磁阻（MR）传感器的官能化的金色图案化薄膜的形状，并同时在传感区域上捕获液滴，先前用氮化硅钝化（Si3N4）。由于与Si3N4相比，由于金的较高的疏水性，液滴保持固定在内部的金框架内，并且精确地实现了传感器的功能化。通过这种策略，我们证明了不同DNA探针和抗体的微分热，以及互补DNA靶和蛋白质的特定杂交和结合，附着于磁珠。这种组合的诱捕和功能化系统在多路复用应用中实现了基于MR的系统的承诺。这项工作也可能感兴趣地在制定其他基于表面的测定的更广泛的社区，也通过微型制造技术开发，因为可以想到与其他生物芯片格式的适应相同的捕获方法。

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  • 来源
    《RSC Advances》 |2017年第68期|共10页
  • 作者

    Dias T. M.; Fernandes E.; Cardoso S.; Monteiro G.; Freitas P. P.;

  • 作者单位

    Inst Nanociencias &

    Nanotecnol INESC Microsyst &

    Nanotechnol Rua Alves Redol P-1000029 Lisbon Portugal;

    Int Iberian Nanotechnol Lab Ave Mestre Jose Veiga P-4715330 Braga Portugal;

    Univ Lisbon Inst Super Tecn Ave Rovisco Pais 1 P-1049001 Lisbon Portugal;

    IBB Ave Rovisco Pais 1 P-1049001 Lisbon Portugal;

    Inst Nanociencias &

    Nanotecnol INESC Microsyst &

    Nanotechnol Rua Alves Redol P-1000029 Lisbon Portugal;

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