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Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells

机译：亲水性细胞衍生的细胞外基质作为促进神经祖细胞的粘附和分化的乳细胞

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摘要

The natural extracellular matrix (ECM) offers a dynamic and intricate microenvironment, which serves as a structural support and regulates cell phenotype and its function. Recently, ECM has been revealed as a favorable and biocompatible architecture for stem cell adhesion and growth in cellular therapy to treat various diseases. However, cell-derived ECM is rarely used as a culture substrate for anchoragedependent cells, such as neural progenitor cells (NPCs), which have the potential to differentiate into basal forebrain cholinergic neurons (BFCNs) for Alzheimer's disease. Here, we report mouse embryonic fibroblast (MEF)-derived ECM, with an appropriately hydrophilic property (water contact angle of 66.8 similar to) to mimic the neural niche for NPCs. In addition, MEF-derived ECM possesses a nanotopological surface, plentiful kinds of components and exhibits excellent adhesion properties for anchoring NPCs. Compared with a laminin-coated plate, MEF-derived ECM promotes NPC proliferation and differentiation into BFCNs by similar to 1.6 fold and similar to 3.1 fold, respectively, consequently enhancing the production of acetylcholine by similar to 2.0 fold. This MEF-derived ECM could be a favorable cell culture carrier for NPC attachment, with great potential for applications in stem cell therapy for Alzheimer's disease.
机译：天然细胞外基质（ECM）提供动态和复杂的微环境，其用作结构支持和调节细胞表型及其功能。最近，ECM已被揭示为干细胞粘附和细胞疗法生长的有利和生物相容性的架构，以治疗各种疾病。然而，细胞衍生的ECM很少用作锚定依赖性细胞的培养基，例如神经祖细胞（NPC），其具有分化为Alzheimer疾病的基础前脑胆碱能神经元（BFCN）。在这里，我们报告小鼠胚胎成纤维细胞（MEF）的ECM，具有适当的亲水性（与66.8的水接触角为66.8）以模仿NPC的神经Niche。此外，MeF衍生的ECM具有纳米腔学，具有丰富种类的组分，并且具有优异的粘合性能以锚定NPC。与层粘连蛋白涂层板相比，MEF衍生的ECM将NPC增殖和分化成BFCNs分别与1.6倍并类似于3.1倍，因此通过类似于2.0倍的2.0倍的增强乙酰胆碱的产生。该MEF衍生的ECM可以是NPC附着的有利细胞培养载体，具有巨大的潜力在干细胞治疗对于阿尔茨海默病的应用。

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  • 来源
    《RSC Advances》 |2017年第72期|共8页
  • 作者

    Yang Lingyan; Jiang Ziyun; Zhou Linhong; Zhao Keli; Ma Xun; Cheng Guosheng;

  • 作者单位

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion CAS Key Lab Nanobio Interface Nanjing 215123 Jiangsu Peoples R China;

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion CAS Key Lab Nanobio Interface Nanjing 215123 Jiangsu Peoples R China;

    Xi An Jiao Tong Univ Sch Med Dept Pharm Xian 710061 Shaanxi Peoples R China;

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion CAS Key Lab Nanobio Interface Nanjing 215123 Jiangsu Peoples R China;

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion CAS Key Lab Nanobio Interface Nanjing 215123 Jiangsu Peoples R China;

    Chinese Acad Sci Suzhou Inst Nanotech &

    Nanobion CAS Key Lab Nanobio Interface Nanjing 215123 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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