DMSO and glycerol reduce bacterial death induced by expression of truncated N-terminal huntingtin with expanded polyglutamine tracts

Yoshiro Nagao; Hiroshi Ishiguro; Nobuyuki Nukina

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DMSO and glycerol reduce bacterial death induced by expression of truncated N-terminal huntingtin with expanded polyglutamine tracts

机译：DMSO和甘油减少了具有扩展的聚谷氨酰胺束的截短的N末端亨廷顿蛋白表达诱导的细菌死亡

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Huntington's disease (HD) is caused by CAG repeat expansion in exon 1 of a large gene, IT15, possessing 67 exons. Transgenic mice expressing a truncated N-terminal peptide of huntingtin with an expanded polyglutamine tract translated only from exon 1 develop symptoms similar to Huntington's disease. In the present study, a bacterial system (Escherichia coli) was used to express truncated peptides of huntingtin translated from exon 1 of the HD gene. Bacterial death was observed after the induction of peptides with expanded polyglutamine tracts, and both sodium dodecyl sulfate (SDS)-soluble peptides and insoluble aggregated material were detected by immunoblotting in the homogenates of such E. coli. E. coli death was partially reduced by the addition of dimethylsulfoxide (DMSO) or glycerol to the medium, with a consequent decrease in aggregated material and an increase in SDS-soluble peptide in the homogenate. These results suggest that DMSO and glycerol may decrease the toxicity of huntingtin with expanded polyglutamine tracts by acting as chemical chaperones.

机译：亨廷顿舞蹈病（HD）是由大基因IT15的外显子1中的CAG重复扩增引起的，该基因具有67个外显子。表达具有仅从外显子1翻译的扩增的聚谷氨酰胺束的亨廷顿蛋白的截短的N-末端肽的转基因小鼠产生类似于亨廷顿氏病的症状。在本研究中，细菌系统（大肠杆菌）用于表达从HD基因第1外显子翻译的亨廷顿蛋白的截短肽。在用扩展的聚谷氨酰胺束诱导肽后观察到细菌死亡，并且通过在此类大肠杆菌的匀浆中进行免疫印迹来检测十二烷基硫酸钠（SDS）可溶性肽和不溶性聚集物质。通过向培养基中添加二甲亚砜（DMSO）或甘油，可部分减少大肠杆菌的死亡，从而减少聚集物质，并增加匀浆中SDS可溶性肽的含量。这些结果表明，DMSO和甘油可通过充当化学分子伴侣来降低具有扩展的聚谷氨酰胺束的亨廷顿蛋白的毒性。

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《Biochimica et biophysica acta. Molecular basis of disease: BBA》 |2000年第2期|共10页
作者
Yoshiro Nagao; Hiroshi Ishiguro; Nobuyuki Nukina;
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正文语种 eng
中图分类生物工程学（生物技术）;
关键词
N-terminal huntingtin; huntingtin; huntingdon's disease; polyglutamine tracts;

机译：N末端亨廷顿蛋白;亨廷顿蛋白;亨廷顿舞蹈病;多谷氨酰胺;

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1. DMSO and glycerol reduce bacterial death induced by expression of truncated N-terminal huntingtin with expanded polyglutamine tracts [J] . Yoshiro Nagao, Hiroshi Ishiguro, Nobuyuki Nukina Biochimica et biophysica acta. Molecular basis of disease: BBA . 2000,第2期

机译：DMSO和甘油减少了具有扩展的聚谷氨酰胺束的截短的N末端亨廷顿蛋白表达诱导的细菌死亡
2. Curcumin enhances the polyglutamine-expanded truncated N-terminal huntingtin-induced cell death by promoting proteasomal malfunction [J] . Dikshit P, Goswami A, Mishra A, Biochemical and Biophysical Research Communications . 2006,第4期

机译：姜黄素通过促进蛋白酶体功能障碍增强多聚谷氨酰胺扩增的截短的N末端亨廷顿蛋白诱导的细胞死亡
3. Altered proteasomal function due to the expression of polyglutamine-expanded truncated N-terminal huntingtin induces apoptosis by caspase activation through mitochondrial cytochrome c release. [J] . Jana NR, Zemskov EA, Gh, Human Molecular Genetics . 2001,第10期

机译：蛋白酶体功能的改变是由于多谷氨酰胺扩展的截短的N末端亨廷顿蛋白的表达引起的，通过线粒体细胞色素c释放的胱天蛋白酶激活来诱导凋亡。
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5. Modulation of inflammatory and T helper cell responses by opioids and IL -4: Direct effects on cytokine production, death effector expression, and activation-induced cell death [D] . Greeneltch, Kristy Markos 2004

机译：阿片类物质和IL -4对炎性和T辅助细胞反应的调节：对细胞因子产生，死亡效应子表达和激活诱导的细胞死亡的直接影响
6. Expanded Polyglutamine-containing N-terminal Huntingtin Fragments Are Entirely Degraded by Mammalian Proteasomes [O] . Katrin Juenemann, Sabine Schipper-Krom, Anne Wiemhoefer, 2013

机译：扩展的包含多聚谷氨酰胺的N端Huntingtin片段被哺乳动物蛋白酶完全降解。
7. DMSO and glycerol reduce bacterial death induced by expression of truncated N-terminal huntingtin with expanded polyglutamine tracts [O] . Nagao Yoshiro, Ishiguro Hiroshi, Nukina Nobuyuki 2000

机译：DMSO和甘油减少了具有扩展的聚谷氨酰胺束的截短的N末端亨廷顿蛋白表达诱导的细菌死亡
8. Hunting for huntingtin associated factors: Identification and characterization of huntingtin expanded polyglutamine aggregate associated factors and their impact on Huntington disease model cellular toxicity. [R] . Wear, M. P. 2016

机译：寻找与亨廷顿相关的因素：亨廷顿扩展的聚谷氨酰胺聚集相关因子的鉴定和表征及其对亨廷顿病模型细胞毒性的影响。

DMSO and glycerol reduce bacterial death induced by expression of truncated N-terminal huntingtin with expanded polyglutamine tracts

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