Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

Heuke Sandro; Sarri Barbara; Lombardini Alberto; Audier Xavier; Rigneault Nerve

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Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

机译：双重聚焦刺激拉曼散射显微镜：多重焦点缩放的概念

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High-speed imaging is of the utmost importance for video-rate live cell investigations or to study extended sample areas at sufficient spatial resolution within reasonable time scales. Improving the speed of single-focus stimulated Raman scattering (SRS) microscopy is ultimately restricted by the sample's damage threshold and the shot noise of the demodulated laser source. To overcome this limitation, we present a dual-focus SRS approach modulating the pump laser for each focus at a distinct frequency. The corresponding probe beams are detected each by a photodiode and demodulated individually by two separate lock-in units to avoid inter-focal cross-talk. Two laterally or axially displaced images as well as hyperspectral SRS images can be obtained simultaneously within the field of view of the objective lens. The modular implementation presented here can be extended to multiple foci by using multi-channel acousto-optics modulators in combination with multi-channel lock-in amplifiers. (C) 2018 Optical Society of America

机译：高速成像对于视频速率直播细胞调查至关重要或在合理的时间尺度内以足够的空间分辨率研究扩展样本区域。提高单焦刺激的拉曼散射（SRS）显微镜的速度最终受样本的损伤阈值和解调激光源的射击噪声限制。为了克服这种限制，我们介绍了一种双对焦SRS方法，用于以不同的频率为每个聚焦的泵激光器调制泵激光器。相应的探针光束被光电二极管检测，并通过两个单独的锁定单元单独解调，以避免焦际串扰。可以在物镜的视场内同时获得两个横向或轴向移位的图像以及高光谱SRS图像。这里呈现的模块化实现可以通过使用多通道声学 - 光学调制器与多通道锁放大器组合使用多通道声音 - 光学调制器来扩展到多个焦点。（c）2018年光学学会

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《Optics Letters》 |2018年第19期|共4页
作者
Heuke Sandro; Sarri Barbara; Lombardini Alberto; Audier Xavier; Rigneault Nerve;
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Aix Marseille Univ Inst Fresnel Cent Marseille CNRS Marseille France;

Aix Marseille Univ Inst Fresnel Cent Marseille CNRS Marseille France;

Aix Marseille Univ Inst Fresnel Cent Marseille CNRS Marseille France;

Aix Marseille Univ Inst Fresnel Cent Marseille CNRS Marseille France;

Aix Marseille Univ Inst Fresnel Cent Marseille CNRS Marseille France;

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正文语种 eng
中图分类计量学;光学;
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专利

1. Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling [J] . Heuke Sandro, Sarri Barbara, Lombardini Alberto, Optics Letters . 2018,第19期

机译：双重聚焦刺激拉曼散射显微镜：多重焦点缩放的概念
2. Molecular Orientational Order Probed by Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) Microscopy: A Spectral Comparative Study [J] . Duboisset Julien, Berto Pascal, Gasecka Paulina, The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2015,第7期

机译：相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS）显微镜探测的分子取向顺序：光谱比较研究。
3. Real-Time Monitoring of Pharmacokinetics of Antibiotics in Biofilms with Raman-Tagged Hyperspectral Stimulated Raman Scattering Microscopy [J] . Kideog Bae, Wei Zheng, Ying Ma, Theranostics . 2019,第5期

机译：拉曼标记的高光谱刺激拉曼散射显微镜实时监测生物膜中抗生素的药代动力学。
4. Effects of refractive index mismatch on stimulated Raman scattering and coherent anti-stokes Raman scattering microscopy [C] . Jarno N. van der Kolk, Antonino Calà Lesina, Lora Ramunno Conference on Lasers and Electro-Optics . 2017

机译：折射率不匹配对受激拉曼散射和相干抗焦炭拉曼散射显微镜的影响
5. Advancements in Stimulated Raman Scattering Microscopy [D] . Figueroa, Benjamin, Jr. 2020

机译：刺激拉曼散射显微镜的进步
6. Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy [O] . Andrew Folick, Wei Min, Meng C. Wang -1

机译：使用相干脂质动力学的无标记成像反斯托克斯拉曼散射（CaRs）和受激拉曼散射（sRs）显微镜
7. High-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering Microscopy [O] . Yasuyuki OZEKI 2017

机译：基于刺激拉曼散射显微镜基于刺激拉曼散射显微镜的刺激拉曼散射微观标记无尺寸标记的无刺激性散射微观标记生物成像的高速标记的光谱散射生物成像

Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling

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