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首页> 外文期刊>Current Microbiology: An International Journal >Genetic variation in clones of Pseudomonas pseudoalcaligenes after ten months of selection in different thermal environments in the laboratory
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Genetic variation in clones of Pseudomonas pseudoalcaligenes after ten months of selection in different thermal environments in the laboratory

机译：在实验室中不同热环境中筛选十个月后，假单胞假单胞菌假单胞菌属克隆的遗传变异

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摘要

The random amplification of polymorphic DNA (RAPD) method was used to examine genetic variation in experimental clones of Pseudomonas pseudoalcaligenes in two experimental groups, as well as their common ancestor. Six clones derived from a single colony of P. pseudoalcaligenes were cultured in two different thermal regimes for 10 months. Three clones in the Control group were cultured at constant temperature of 35 degrees C and another three clones in the High Temperature (HT) group were propagated at incremental temperature ranging from 41 to 47 degrees C for 10 months. A total of 45 RAPD primers generated 146 polymorphic markers. Analysis of molecular variance (AMOVA) revealed mild (11%) but significant (P < 0.001) genetic difference between the Control and the HT clones. Phylogenetic analysis based on pairwise genetic distances showed that the HT clones were more divergent from the ancestor and from each other than the Control clones, implying that the HT clones of P. pseudoalcaligenes may have evolved faster than the Control clones.
机译：多态性DNA随机扩增（RAPD）方法用于检查两个实验组中假拟假单胞菌的实验克隆及其共同祖先的遗传变异。在两个不同的热疗条件下，培养了来自假拟青假单胞菌单个菌落的六个克隆，共培养了10个月。对照组中的三个克隆在35摄氏度的恒温下培养，高温（HT）组中的另外三个克隆在41至47摄氏度的增量温度下繁殖10个月。共有45种RAPD引物产生146种多态性标记。分子变异分析（AMOVA）显示，对照和HT克隆之间存在轻度（11％）但显着（P <0.001）的遗传差异。基于成对遗传距离的系统发育分析表明，HT克隆与对照克隆相比，祖先和彼此之间的分歧更大，这意味着假拟假丝酵母的HT克隆的进化可能比对照克隆快。

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