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首页> 外文期刊>Molecular Microbiology >A novel two‐component system modulates quorum sensing and pathogenicity in Burkholderia cenocepacia Burkholderia cenocepacia
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A novel two‐component system modulates quorum sensing and pathogenicity in Burkholderia cenocepacia Burkholderia cenocepacia

机译：一种新型的双组分系统在Burkholderia Cenocepacia Burkholderia Cenocepacia调节了Quorum感测和致病性

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摘要

Summary Quorum sensing (QS) is widely utilized by bacterial pathogens to regulate biological functions and pathogenicity. Recent evidence has shown that QS is subject to regulatory cascades, especially two‐component systems that often respond to environmental stimulation. At least two different types of QS systems regulate pathogenesis in Burkholderia cenocepacia . However, it remains unclear how this bacterial pathogen controls these QS systems. Here, we demonstrate a novel two‐component system RqpSR ( R egulating Q uorum sensing and P athogenicity), which plays an important role in modulating QS and pathogenesis in B. cenocepacia . We demonstrate strong protein‐protein binding affinity between RqpS and RqpR. Mutations in rqpS and rqpR exerted overlapping effects on B. cenocepacia transcriptomes and phenotypes, including motility, biofilm formation and virulence. In trans expression of rqpR rescued the defective phenotypes in the rqpS mutant. RqpR controls target gene expression by direct binding to DNA promoters, including the cis ‐2‐dodecenoic acid (BDSF) and N ‐acylhomoserine lactone (AHL) signal synthase gene promoters. These findings suggest that the RqpSR system strongly modulates physiology by forming a complicated hierarchy with QS systems. This type of two‐component system appears to be widely distributed and coexists with the BDSF QS system in various bacterial species.
机译：发明内容Quorum感测（QS）被细菌病原体广泛利用以调节生物学功能和致病性。最近的证据表明，QS受到监管级联，特别是经常响应环境刺激的双组分系统。至少两种不同类型的QS系统调节Burkholderia Cenocepacia的发病机制。然而，仍然尚不清楚这种细菌病原体如何控制这些QS系统。在这里，我们证明了一种新型的双组分系统RQPSR（Regulate Quorum感测和P antogensicity），其在调节Qs和B. cenocepacia中的发病机制起着重要作用。我们在RQP和RQPR之间展示了强蛋白质 - 蛋白质结合亲和力。 RQPS和RQPR中的突变对B. cenocepacia转录om和表型施加重叠效应，包括运动，生物膜形成和毒力。在RQPR的反式表达中，RQPS突变体中的缺陷表型。 RQPR通过直接结合DNA启动子来控制靶基因表达，包括顺式-2-十二烷烯酸（BDSF）和N-acylhomoserine内酯（AHL）信号合酶基因启动子。这些发现表明RQPSR系统通过形成具有QS系统的复杂层次结构强烈调节生理学。这种类型的双组分系统似乎广泛分布并在各种细菌种类中与BDSF QS系统共存。

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  • 来源
    《Molecular Microbiology》 |2018年第1期|共13页
  • 作者

    Cui Chaoyu; Yang Chunxi; Song Shihao; Fu Shuna; Sun Xiuyun; Yang Liang; He Fei; Zhang Lian‐Hui; Zhang Yongliang; Deng Yinyue;

  • 作者单位

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    Singapore Centre for Environmental Life Sciences Engineering (SCELSE)Nanyang Technological;

    Integrative Microbiology Research CentreSouth China Agricultural UniversityGuangzhou 510642 China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

    Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University of;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro‐BioresourcesSouth China;

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  • 正文语种 eng
  • 中图分类 细胞生物学;
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