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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >A new nutraceutical resource from a rare native plant growing in Turkey and for its spectro-chemical and biological insights: Endemic Diplotaenia bingolensis (Apiaceae)
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A new nutraceutical resource from a rare native plant growing in Turkey and for its spectro-chemical and biological insights: Endemic Diplotaenia bingolensis (Apiaceae)

机译：来自土耳其生长的稀有土着植物的新营养素质资源，并为其光谱 - 化学和生物见解：特点兼论宾果（Apiaceae）

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The present study designed to investigate the quantitative distributions in the secondary metabolites and biological activity of sub-fractions obtained successively from water and methanol: dichloromethane ( rate 1:1: v:v) solvent decoction of Diplotenia bingolensis aerial parts. The crude extracts were obtained from the aerial parts of the endemic D. bingolensis species refluxing with water and organic solvents. Sub-fractions of water extract were obtained by successive fractionation of the water extract with hexane (WH), dichloromethane (WD), ethyl acetate (WE) and n-butanol (WB), respectively. Sub-fractions of organic solvent were obtained by fractionation of the organic crude extract with hexane (OH), dichloromethane (OD), ethyl acetate (OE), n-butanol (OB) and water (OW), respectively. The total amount of phenols and flavonoids contained in each sub-fraction was analyzed by UV-VIS spectrophotometer, analysis of lipophil ic components by GC-MS spectrometer, and quantitative analysis of hydrophilic components by HPLC-TOF/MS spectrophotometer. Furthermore, the biological activity of each sub-fraction was compared with different antioxidant activities such as DPPH radical scavenging activity and ferric ion reduction capacity. Sub-fraction WD (137.1 +/- 2.1 mu g QE/mg DI) and OE (127.1 +/- 52 mu g QE/mg DI) in terms of flavonoid content, sub-fraction WD (665.8 = 47.6 mu g GAE/mg DI) and OE (724.6 +/- 43.6 mu g GAE/mg DI) were the richest isolates in terms of total phenol content. Sub-fractions OH and OD contained linoleic acid (17.0 and 11.0%, respectively) and linolenic acid (22.1 and 18.5%, respectively). It was revealed that sub-fractions were rich in terms of rutin (1.2-472 mu g HC/mg DI) and chlorogenic acid (0.1-12.1 mu g HC/mg DI). Sub-fractions WD and OE were showed the highest DPPH radical scavenging activity with 46.4 +/- 1.4 and 47.6 +/- 10.0 mu g/mL EC 50 values, respectively. This study is the first to demonstrate biological insight of the potential ant
机译：本研究旨在研究次级代谢物中的定量分布，以及从水和甲醇所获得的亚级分的生物活性：二氯甲烷（速率1：1：V：V）溶剂汤，Diplotenia Bingolensis Acial零件。粗提取物是从单体D.弯曲物种与水和有机溶剂回流的弯曲物种。通过用己烷（WH），二氯甲烷（WD），乙酸乙酯（We）和正丁醇（WB）的水提取物的连续分离，得到水提取物的亚级分。通过分离己烷（OH），二氯甲烷（OD），乙酸乙酯（OE），正丁醇（OB）和水（OW）的有机粗提取物的分馏得到有机溶剂的亚级分。通过UV-Vis分光光度计分析每种亚部分中含有的酚类和黄酮类化合物的总量，通过GC-MS光谱仪分析脂石IC组分，以及通过HPLC-TOF / MS分光光度计进行亲水组分的定量分析。此外，将每个亚级分的生物活性与不同的抗氧化活性进行比较，例如DPPH自由基清除活性和铁离子降低能力。在类黄酮含量，子级分（665.8 =47.6μg/）中，亚级（137.1 +/-2.1μg/ mg di）和Oe（127.1 +/-52μmg qe / mg di）（665.8 =47.6μg/ Mg di）和Oe（724.6 +/-43.6μggae / mg di）是在总苯酚含量方面是最富有的分离物。亚级分OH和OD含有亚油酸（分别为17.0-11.0％）和亚麻酸（分别分别为22.1和18.5％）。揭示亚级分富含芦丁（1.2-472μg/ mg二）和绿原酸（0.1-12.1μg/ mg di）。亚级分和OE分别显示出具有46.4 +/- 1.4和47.6 +/-10.0μmG/ mL EC 50值的最高DPPH自由基清除活性。本研究是第一个展示潜在蚂蚁的生物洞察力的人

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