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首页> 外文期刊>Surgical Endoscopy >Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine.
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Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine.

机译：血肿性腹膜腹膜腹膜腹泻胞质梗死的影响：猪的实验研究。

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One of the main concerns of natural orifice surgery is the local and systemic impact on physiology. Few studies have compared natural orifice transluminal endoscopic surgery (NOTES) with other surgical modalities. Most studies are based on systemic variables such as postoperative serum cytokines, with conflicting results. Surgical trauma induces an early inflammatory response, release of cytokines, and local leukocyte activation and oxidative burst. Major surgical trauma is related to impairment of phagocytic function and an increase in production of active oxygen species by phagocytes. The aim of this study was to evaluate the impact of transgastric peritoneoscopy on peritoneal innate immune response compared with laparoscopy and laparotomy in swine.Thirty-four male Sus scrofa domesticus swine were assigned to four groups: transgastric peritoneoscopy (13), laparoscopy (7), laparotomy (7), and sham procedure (7). Twenty-four hours after the procedure, peritoneal fluid cells were harvested by peritoneal washing after necropsy. Flow cytometry analysis of labeled S. aureus and E. coli phagocytosis by peritoneal neutrophils and macrophages was blindly performed. Oxidative burst activity measured by H(2)O(2) production under different challenges was also evaluated.Total operative time varied between all groups. The transgastric, laparoscopy, and laparotomy groups required 56, 17.2, and 40.3 min of mean operative time, respectively (p < 0.05). Even though the mean percentage and intensity of phagocytosis by peritoneal phagocytes were higher in the sham, transgastric, and laparoscopy groups, there was no significant difference between these groups and laparotomy. Macrophage production of H(2)O(2) has been shown to be similar among the transgastric, laparoscopy, and sham groups, and smaller than that in laparotomy (p < 0.05), either under basal conditions, while performing E. coli phagocytosis, or challenged by the presence of E. coli membrane lipopolysaccharide.Under the conditions of this study, transgastric peritoneoscopy has been shown to have minimal impact on peritoneal innate immune response.
机译：天然孔窗手术的主要问题之一是对生理学的局部和全身的影响。少量研究与其他外科样片进行了与其他手术方式进行了比较了天然孔口分子内窥镜手术（注意事项）。大多数研究基于术后血清细胞因子等系统性变量，结果相互矛盾。手术创伤诱导早期炎症反应，细胞因子释放，以及局部白细胞活化和氧化爆发。主要手术创伤与吞噬功能的损害有关，吞噬细胞产生活性氧物种的增加。该研究的目的是评估血肿和腹腔镜和猪的腹腔镜和剖腹术相比血管内腹膜镜检查对腹膜腹膜的影响。四次男性SUS Scrofa Domeiveus Swine分配给四组：血管科静脉检查室（13），腹腔镜（7） ，剖腹手术（7）和假手术（7）。手术后二十四小时，尸检后腹膜洗涤收获腹膜液细胞。盲目地进行了标记的S.UUREUS和大肠杆菌吞噬作用的流式细胞术分析。通过H（2）O（2）在不同挑战下产生的氧化突发活性也在不同挑战下进行评估。所有组之间的操作时间变化。平均手术时间的平均术血管分段，腹腔镜和剖腹手术组分别需要56,17.2和40.3分钟（P <0.05）。尽管假神经植物和腹腔镜术中的腹膜吞噬细胞的平均百分比和吞噬作用的强度较高，但这些组和剖腹手术之间没有显着差异。 H（2）o（2）的巨噬细胞产生在综胎，腹腔镜检查和假组中具有相似，并且小于基础条件下的剖腹产术（P <0.05）中的相似，同时表现为大肠杆菌吞噬作用或者通过大肠杆菌膜脂多糖的存在挑战。该研究的条件下，已显示综痛性腹膜检查术对腹膜腹泻的免疫反应影响最小。

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