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首页> 外文期刊>Surgical Endoscopy >Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine.
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Effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine.

机译：经胃腹膜通路对腹膜先天细胞免疫的影响：猪的实验研究。

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One of the main concerns of natural orifice surgery is the local and systemic impact on physiology. Few studies have compared natural orifice transluminal endoscopic surgery (NOTES) with other surgical modalities. Most studies are based on systemic variables such as postoperative serum cytokines, with conflicting results. Surgical trauma induces an early inflammatory response, release of cytokines, and local leukocyte activation and oxidative burst. Major surgical trauma is related to impairment of phagocytic function and an increase in production of active oxygen species by phagocytes. The aim of this study was to evaluate the impact of transgastric peritoneoscopy on peritoneal innate immune response compared with laparoscopy and laparotomy in swine.Thirty-four male Sus scrofa domesticus swine were assigned to four groups: transgastric peritoneoscopy (13), laparoscopy (7), laparotomy (7), and sham procedure (7). Twenty-four hours after the procedure, peritoneal fluid cells were harvested by peritoneal washing after necropsy. Flow cytometry analysis of labeled S. aureus and E. coli phagocytosis by peritoneal neutrophils and macrophages was blindly performed. Oxidative burst activity measured by H(2)O(2) production under different challenges was also evaluated.Total operative time varied between all groups. The transgastric, laparoscopy, and laparotomy groups required 56, 17.2, and 40.3 min of mean operative time, respectively (p < 0.05). Even though the mean percentage and intensity of phagocytosis by peritoneal phagocytes were higher in the sham, transgastric, and laparoscopy groups, there was no significant difference between these groups and laparotomy. Macrophage production of H(2)O(2) has been shown to be similar among the transgastric, laparoscopy, and sham groups, and smaller than that in laparotomy (p < 0.05), either under basal conditions, while performing E. coli phagocytosis, or challenged by the presence of E. coli membrane lipopolysaccharide.Under the conditions of this study, transgastric peritoneoscopy has been shown to have minimal impact on peritoneal innate immune response.
机译：自然孔口手术的主要关注之一是对生理的局部和全身性影响。很少有研究将自然孔腔内镜手术（NOTES）与其他手术方式进行比较。大多数研究都是基于全身变量，如术后血清细胞因子，结果相互矛盾。手术创伤会引起早期炎症反应，细胞因子释放以及局部白细胞活化和氧化性爆发。重大外科手术创伤与吞噬功能受损和吞噬细胞活性氧种类产生增加有关。这项研究的目的是评估与腹腔镜检查和剖腹手术相比，经胃腹膜镜检查对猪腹膜先天免疫反应的影响。将34只雄性Sus scrofa domesticus猪分为四组：经胃腹膜镜检查（13），腹腔镜检查（7） ，剖腹手术（7）和假手术（7）。手术后二十四小时，尸检后通过腹膜冲洗收集腹膜液细胞。盲目进行了腹膜中性粒细胞和巨噬细胞对标记的金黄色葡萄球菌和大肠杆菌吞噬作用的流式细胞术分析。还评估了在不同挑战下通过H（2）O（2）生产测得的氧化爆发活性。所有组之间的总手术时间不同。经胃，腹腔镜和剖腹手术组分别需要56、17.2和40.3分钟的平均手术时间（p <0.05）。即使假手术组，经胃检查组和腹腔镜检查组腹膜吞噬细胞吞噬作用的平均百分比和强度较高，但这些组和剖腹手术之间没有显着差异。 H（2）O（2）的巨噬细胞产生已被证明在胃，腹腔镜和假手术组之间相似，并且比在基础条件下进行大肠杆菌吞噬作用时的剖腹术要小（p <0.05）。或受到大肠杆菌膜脂多糖的挑战。在这项研究的条件下，经胃腹膜镜检查已显示出对腹膜先天免疫反应的影响最小。

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