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首页> 外文期刊>Physical chemistry chemical physics: PCCP >Understanding and improving aggregated gold nanoparticle/dsDNA interactions by molecular spectroscopy and deconvolution methods
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Understanding and improving aggregated gold nanoparticle/dsDNA interactions by molecular spectroscopy and deconvolution methods

机译：通过分子光谱和去卷积方法理解和改善聚集的金纳米粒子/ DSDNA相互作用

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摘要

It is well known that single-stranded DNA (ssDNA) is easily able to adsorb on citrate-capped, nonfunctionalized gold nanoparticles (AuNPs). However, the affinity of double-stranded DNA (dsDNA) for them is much more limited. The present work demonstrates that long dsDNA suffers from a bending conformational change when anionic nanoparticles are present in solution. A striking decrease in the persistence length of the double helix in the absence of salt is observed through dynamic light scattering (DLS), viscometric, and atomic force microscopy (AFM) methods. Long dsDNA is therefore shown to be able to interact with anionic gold nanoparticles. To date, only ssDNA detection has been described by making use of interparticle cross-linking aggregation mechanisms; however, the data shown in this work allow for the development of new methods for detecting dsDNA in solution by using aggregated AuNPs as a starting point. The aggregation state is induced by the controlled addition of an inert electrolyte. A deconvolution procedure of the experimental plasmon shows how individual bands corresponding to aggregated nanoclusters diminish as the DNA concentration increases in the presence of 0.075 M NaCl.
机译：众所周知，单链DNA（SSDNA）易于吸附在柠檬酸盐的非官能化金纳米颗粒（AUNP）上吸附。然而，双链DNA（DSDNA）对它们的亲和力更有限。本作者表明，当阴离子纳米颗粒存在于溶液中时，长DSDNA患有弯曲构象变化。通过动态光散射（DLS），粘度测定和原子力显微镜（AFM）方法，观察到在没有盐的情况下的双螺旋持续长度的引人注目的降低。因此，LONG DSDNA能够与阴离子金纳米颗粒相互作用。迄今为止，仅通过使用颗粒体交联聚集机制来描述SSDNA检测;然而，本工作中所示的数据允许通过使用聚合的AUNP作为起点，开发用于在解决方案中检测DSDNA的新方法。通过受控添加惰性电解质诱导聚集状态。实验性等离子体的解卷积过程表明，随着DNA浓度在0.075M NaCl的存在下增加，当DNA浓度增加时，对应于聚集的纳米团簇的单个带。

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