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Construction and functional validation of a new promoter-probe vector pRR21

机译：新的启动子探针载体pRR21的构建和功能验证

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摘要

The promoter, a DNA sequence normally located upstream of the transcribed region, plays an important role in controlling transgene activity. Choice of the promoter to confer constitutive, spatial and/or temporal transgene expression is one of the key determinants in plant biotechnology applications. In recent years, a wide range of different promoters from plant, viral and bacterial origin have been characterized and used extensively in regulating transgene expression in plants. However, most of them are protected by patents. Therefore, novel plant sequences that can function as promoters for transgene expression are to be identified and tested. A wider range of effective promoters would also make it possible to introduce multiple transgenes into plant cells while still avoiding the risk of homology-dependent gene silencing.
机译：启动子是通常位于转录区上游的DNA序列，在控制转基因活性中起重要作用。赋予组成型，空间和/或时间性转基因表达的启动子的选择是植物生物技术应用中的关键决定因素之一。近年来，已表征了来自植物，病毒和细菌的多种不同启动子，并广泛用于调节植物中的转基因表达。但是，其中大多数受专利保护。因此，将鉴定和测试可用作转基因表达的启动子的新型植物序列。有效启动子的范围更广，也有可能将多个转基因导入植物细胞，同时仍避免同源依赖性基因沉默的风险。

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