Cloning of mineral phosphate solubilizing genes from Synechocystis PCC 6803

Gyaneshwar P.; Parekh LJ.; Kumar GN.

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Cloning of mineral phosphate solubilizing genes from Synechocystis PCC 6803

机译：拟南芥PCC 6803的矿物磷酸盐增溶基因的克隆

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Genes responsible for conferring mineral phosphate solubilizing (mps) ability were cloned from Synechocystis PCC 6803, a unicellular cyanobacterium, which by itself does not show mps ability. Escherichia coli was transformed with the genomic DNA library of the Synechocystis PCC 6803 and two mps genes were selected based on the ability of the transformants to show zone of clearance on solid medium containing dicalcium phosphate (DCP) as the sole source of phosphorus (P). The mps phenotype was found to be plasmid-associated as shown by transformation of E. coli strain with isolated plasmids. These transformants also solubilized rock phosphate. [References: 12]

机译：从单胞藻蓝藻集胞藻PCC 6803中克隆了赋予矿物磷酸盐增溶（mps）能力的基因，它本身不显示mps能力。用集胞蓝藻PCC 6803的基因组DNA文库转化大肠杆菌，并根据转化子在包含磷酸二钙（DCP）作为唯一磷（P）的固体培养基上显示清除区的能力，选择了两个mps基因。发现mps表型与质粒相关，如通过用分离的质粒转化大肠杆菌菌株所显示的。这些转化体也溶解了磷酸岩。 [参考：12]

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《Current Science: A Fortnightly Journal of Research》 |1998年第12期|共3页
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Gyaneshwar P.; Parekh LJ.; Kumar GN.;
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1. Cloning of mineral phosphate solubilizing genes from Synechocystis PCC 6803 [J] . Gyaneshwar P., Parekh LJ., Kumar GN. Current Science: A Fortnightly Journal of Research . 1998,第12期

机译：拟南芥PCC 6803的矿物磷酸盐增溶基因的克隆
2. Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization [J] . Patra B, Dastidar KG, Maitra S, Planta: An International Journal of Plant Biology . 2007,第6期

机译：蓝藻属PCC 6803中sll1383作为L-肌醇1-磷酸磷酸酶（EC 3.1.3.25）的功能鉴定：分子克隆，表达与表征
3. Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization [J] . Barunava Patra, Krishnarup Ghosh Dastidar, Susmita Maitra, Planta . 2007,第6期

机译：蓝藻属PCC 6803中sll1383作为L-肌醇1-磷酸磷酸酶的功能鉴定（EC 3.1.3.25）：分子克隆，表达与表征
4. Acid Stress Responsive Genes, slr0967 and sll0939, are Directly Involved in Low-pH Tolerance of Cyanobacterium Synechocystis sp. PCC6803. [C] . Hisataka Ohta, Yuta Kobayashi, Atsushi Moriyama, Photosynthesis research for food, fuel and future . 2010

机译：酸性胁迫响应基因slr0967和sll0939直接参与蓝藻蓝藻的低pH耐受性。 PCC6803。
5. Expression of heat shock genes hsp16.6 and htpG in the cyanobacterium, Synechocystis sp. PCC 6803 [D] . Fang, Feng 2003

机译：热激基因hsp16.6和htpG在蓝细菌Synechocystis sp中的表达。 PCC 6803
6. Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis strain PCC 6803. [O] . J Labarre, F Chauvat, P Thuriaux 1989

机译：通过将抗生素抗性基因随机克隆到蓝藻集胞藻菌株PCC 6803的基因组中进行插入诱变。
7. A putative cytochrome c biogenesis gene in Synechocystis sp. PCC 68031The nucleotide sequence of orf334 from Synechocystis sp. PCC 6803 appeared in EMBL and GenBank under accession No. Z72480.1 [O] . Hübschmann Thomas, Wilde Annegret, Elanskaya Irina, 1997

机译：假定的细胞色素c生物发生基因在Synechocystis sp.。 PCC 68031来自集胞藻属（Synechocystcystis sp。）的orf334的核苷酸序列。 PCC 6803出现在EMBL和GenBank中，登录号为Z72480.1

Cloning of mineral phosphate solubilizing genes from Synechocystis PCC 6803

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