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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Rachilla: an additional source for embryogenic callus induction and transformation in Oryza sativa L.
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Rachilla: an additional source for embryogenic callus induction and transformation in Oryza sativa L.

机译：Rachilla：水稻中胚性愈伤组织诱导和转化的另一个来源。

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摘要

Rachilla, the portion joining sterile glumes to palea and lemma, in the rice spikelet was found to be an additional source of embryogenic callus for culture and transformation. Induction of callus from rachilla was distinct in inflorescences of size3 cm or above. Inflorescence length ranging between 3 and 5 cm was found to be ideal for induction of callus from rachilla. The response was optimum when the florets were of 2-5 mm. The callus induced from rachilla is lustrous, friable and embryogenic,and facilitated much faster establishment of uniform embryogenic suspension cultures (3-4 weeks) compared to seed-derived calli (6-8 weeks). Though the frequency of callus induction from rachilla is low compared to mature seed-derived calli, the regeneration potential of the former is higher. The rachilla callus was used for bombardment either directly or after establishment of suspension cultures. The calli derived from rachilla did show advantage over seed-derived calli by hastening the process of establishing suspension cultures or for genetic transformation.
机译：在水稻小穗中，Rachilla是将无菌颖片连接至pala和lemma的部分，被发现是用于培养和转化的胚发生愈伤组织的另一来源。从山chill的愈伤组织的诱导在3厘米或以上的花序中是明显的。发现花序长度在3至5cm之间是理想的用于诱导来自山a的愈伤组织。当小花为2-5毫米时，响应最佳。与种子衍生的愈伤组织（6-8周）相比，由罗奇拉诱导的愈伤组织有光泽，易碎且具有胚发生性，并有助于更快地建立统一的胚发生悬浮培养物（3-4周）。尽管与成熟的种子衍生的愈伤组织相比，罗吉拉的愈伤组织诱导频率低，但前者的再生潜力更高。树chill愈伤组织直接或在悬浮培养物建立后用于轰击。通过加快建立悬浮培养物或进行遗传转化的过程，源自山chill的愈伤组织确实显示出优于源自种子的愈伤组织的优势。

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