首页> 外文期刊>Current Science: A Fortnightly Journal of Research >CYTOKININS AND IN VITRO INDUCTION OF FLOWERING IN BAMBOO - BAMBUSA ARUNDINACEA (RETZ.) WILLD
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CYTOKININS AND IN VITRO INDUCTION OF FLOWERING IN BAMBOO - BAMBUSA ARUNDINACEA (RETZ.) WILLD

机译:竹子中的细胞分裂素和花粉的体外诱导

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Four-week-old seedling explants of Bambusa arundinacea were cultured on Murashige and Skoog (MS) basal medium with different cytokinins to study the effect on in vitro induction of flowering, The cytokinins used were adenine hemisulphate (AdS), 6-[gamma,gamma-dimethylallylamino]-purine (2iP), kinetin (Kin), zeatin individually and in combination with 6-benzyl adenine (BA), BA was found to be the only cytokinin inducing flowering in vitro when added individually in the culture medium. The induction of flowering ill media containing other cytokinins, when added individually, was not observed, However, induction of flowering was observed in all the cytokinin combinations whenever BA was added, Presence of BA in the culture medium was observed to be absolutely essential for induction of flowering. 2iP showed synergistic effect in combination with BA although it was ineffective in the presence of flower induction alone, while zeatin showed antagonistic effect on induction of flowering in combination with BA. [References: 21]
机译:在具有不同细胞分裂素的Murashige和Skoog(MS)基础培养基上培养了四周长的Bambusa arundinacea幼苗外植体,以研究其对体外诱导开花的影响。所用的细胞分裂素为腺嘌呤半硫酸盐(AdS),6- [γ, γ-二甲基烯丙基氨基]嘌呤(2iP),激动素(Kin),玉米蛋白分别与6-苄基腺嘌呤(BA)结合使用,发现BA是单独添加到培养基中的唯一诱导离体开花的细胞分裂素。单独添加时,未观察到含有其他细胞分裂素的开花不良培养基的诱导,但是,每当添加BA时,在所有细胞分裂素组合中均观察到开花诱导,观察到培养基中BA的存在对于诱导绝对必要开花。 2iP与BA组合显示协同作用,尽管仅在诱导花的情况下无效,而玉米蛋白与BA组合对诱导开花的拮抗作用。 [参考:21]

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