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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Algal biofuel: bountiful lipid from Chlorococcum sp. proliferating in municipal wastewater.
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Algal biofuel: bountiful lipid from Chlorococcum sp. proliferating in municipal wastewater.

机译：藻类生物燃料：绿球藻的丰富脂质。在城市废水中扩散。

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摘要

Algae biofuel have emerged as viable renewable energy sources and are the potential alternatives to fossil-based fuels in recent times. Algae have the potential to generate significant quantities of commercially viable biofuel apart from treating wastewater. Three algal species, viz. Chlorococcum sp., Microcystis sp. and Phormidium sp. proliferating in wastewater ponds were isolated and cultured in the laboratory myxotrophically under similar wastewater conditions. Chlorococcum sp. attained a mean biomass productivity of 0.09 g l-1 d-1 with the maximum biomass density of 1.33 g l-1 and comparatively higher lipid content of 30.55% (w/w) on the ninth day of the culture experiment. Under similar conditions Microcystis sp. and Phormidium sp. attained mean biomass productivities of 0.058 and 0.063 g l-1 d-1 with a total lipid content of 8.88% and 18.66% respectively. Biochemical composition (carbohydrates, proteins, lipids and phosphates) variations and lipid accumulation studies were performed by comparison of the ratios of carbohydrate to protein; lipid to protein (L/P) and lipid to phosphates using attenuated total reflectance-Fourier transform infrared spectroscopy which showed higher L/P ratio during the stationary phase of algal growth. Composition analysis of fatty acid methyl ester has been performed using gas chromatography and mass spectrometry. Chlorococcum sp. with higher productivity and faster growth rate has higher lipid content with about 67% of saturated fatty acid dominated by palmitate (36.3%) followed by an unsaturate as linoleate (14%) and has proved to be an economical and viable feedstock for biofuel production compared to the other wastewater-grown species.
机译：藻类生物燃料已成为可行的可再生能源，并且是近代化石燃料的潜在替代品。除处理废水外，藻类还可能产生大量商业上可行的生物燃料。三种藻类，即。绿球藻属，微囊藻属和Phormidium sp。在相似的废水条件下，分离并在实验室中进行营养培养，分离出废水中增殖的池塘。绿球藻达到平均生物量生产力0.09 gl -1 d -1 的最大生物量密度为1.33 gl -1 和较高的脂质含量培养实验第9天时为30.55％（w / w）。在相似条件下，微囊藻和Phormidium sp。分别获得了0.058和0.063 g l -1 d -1 的平均生物生产力，总脂质含量分别为8.88％和18.66％。通过比较碳水化合物与蛋白质的比例，进行了生化成分（碳水化合物，蛋白质，脂质和磷酸盐）的变化和脂质蓄积研究。使用衰减全反射-傅立叶变换红外光谱法测定脂质与蛋白质（L / P）和脂质与磷酸盐，在藻类生长的稳定期显示出较高的L / P比。脂肪酸甲酯的组成分析已使用气相色谱法和质谱法进行。绿球藻具有较高的生产率和更快的生长速度，具有较高的脂质含量，其中约67％的饱和脂肪酸以棕榈酸酯为主（36.3％），其次是不饱和的亚油酸酯（14％），与生物燃料生产相比，它是经济且可行的原料到其他废水中生长的物种。

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