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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Genetic transformation of Terminalm chebula Retz. and detection of tannin in transformed tissue
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Genetic transformation of Terminalm chebula Retz. and detection of tannin in transformed tissue

机译：Terminalm chebula Retz的遗传转化。转化组织中单宁的合成与检测

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Genetic transformation of Terminalia chebula Retz. was carried out using Agrobacterium tumefaciens strain C-58. Explants such as cotyledon, hypocotyl, excised mature zygotic embryo, cotyledonary node, in vitro leaf and shoot were used for genetic transformation. Different experimental methods were followed for infecting the explants. Cotyledon and hypocotyl explants showed swelling response on MS basal medium subsequent to genetic transformation. About 32.5 +- 2.5 % cultures showed swelling responsewhen 8-12 d in vitro pre-cultured cotyledon explants were used. Swelling response was not observed in non-transformed control cotyledon explants. Callus induction was observed in one of the swollen cotyledon explants infected with the bacterial suspension grown on media containing 0.1 mM acetosyringone. Callus initiation was not observed in cotyledon explants without co-cultivation, which were kept as control. The transformed callus was subjected to nopaline assay using paper electro-phoresis. The analysis indicated the transformed nature of the callus with the presence of nopaiine and its absence in non-transformed control callus. Transformed callus grown on fresh MS basal medium showed more than two-fold increase in the growth after four weeks of culture compared to normal control callus. Normally no growth was observed in imtransformed control callus. The transformed callus was analysed for the presence of tannins using thin layer chromato-graphy, which indicated the presence of tannic acid in thetransformed callus. Genetic transformation of T. chebula and detection of tannin in transformed callus are reported here. This can be used to study the tannin biosynthetic pathway using biochemical and molecular approach.
机译：榄仁Terminal的遗传转化。使用根癌土壤杆菌菌株C-58进行。外植体如子叶，下胚轴，切下的成熟合子胚，子叶节，离体叶和芽用于遗传转化。遵循不同的实验方法感染外植体。子叶和下胚轴外植体在遗传转化后在MS基础培养基上显示出肿胀反应。当使用体外预培养的子叶外植体8-12 d时，约32.5±2.5％的培养物表现出溶胀反应。在未转化的对照子叶外植体中未观察到溶胀反应。在感染了在含有0.1 mM乙酰丁香酮的培养基上生长的细菌悬液的一种肿胀的子叶外植体中观察到愈伤组织诱导。未经共培养的子叶外植体未观察到愈伤组织的萌生，将其作为对照。使用纸电泳对转化的愈伤组织进行胭脂碱测定。分析表明，存在胭脂碱和未转化的对照愈伤组织中不存在胭脂氨酸的愈伤组织具有转化性质。与正常对照愈伤组织相比，在新鲜的MS基础培养基上生长的转化愈伤组织在培养4周后显示出两倍以上的生长增长。通常在未转化的对照愈伤组织中未观察到生长。使用薄层色谱法分析了转化的愈伤组织中单宁的存在，这表明转化的愈伤组织中单宁酸的存在。这里报道了T. chebula的遗传转化和转化愈伤组织中单宁的检测。这可以用于利用生化和分子方法研究单宁生物合成途径。

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