Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers

Bhuyan Nipon; Borah Basanta K.; Sarma R. N.

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Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers

机译：利用RAPD和ISSR标记分析阿萨姆邦传统低地稻（Oryza sativa L.）的遗传多样性。

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Genetic variability among 24 rice genotypes from Assam was assessed employing random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) using ten primers in each case. A total of 81 RAPD and 201 ISSR markers were generated with 92.5 and 98% polymorphism respectively. The average polymorphism information content (PIC) for RAPD was 0.741 and for ISSR markers it was 0.888. The Jaccard's similarity coefficient based on RAPD and ISSR analysis was 0.480 and 0.257 respectively. Cluster analysis and cophenetic correlation value based on the ISSR data discretely separated the accessions, according to farmer's classification. This communication reports genetic variability in rice from Assam using two molecular markers.

机译：使用随机扩增多态性DNA（RAPD）和简单序列间重复聚合酶链反应（ISSR-PCR）使用十种引物评估来自阿萨姆邦的24种水稻基因型的遗传变异性。总共产生了81个RAPD和201个ISSR标记，分别具有92.5和98％的多态性。 RAPD的平均多态信息含量（PIC）为0.741，ISSR标记的平均多态信息含量为0.888。基于RAPD和ISSR分析的Jaccard相似系数分别为0.480和0.257。根据农民的分类，基于ISSR数据的聚类分析和同感相关值将种质分离。该通报报道了使用两种分子标记的阿萨姆邦水稻的遗传变异性。

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《Current Science: A Fortnightly Journal of Research》 |2007年第7期|共6页
作者
Bhuyan Nipon; Borah Basanta K.; Sarma R. N.;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
genotype; genetic diversity; genetic variability; plant accession;

机译：基因型;遗传多样性;遗传变异性;植物登录;

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1. Identification of a RAPD marker linked to a blast resistance gene in Oryza sativa L. [J] . LUJun, ZHUANGJieyun, LINHongxuan, 中国水稻研究通报：英文版 . 1994,第004期
2. Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. [J] . 严成其, 钱凯先, 薛刚平, 浙江大学学报：自然科学英文版 . 2004,第010期
3. Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers [J] . Bhuyan Nipon, Borah Basanta K., Sarma R. N. Current Science: A Fortnightly Journal of Research . 2007,第7期

机译：利用RAPD和ISSR标记分析阿萨姆邦传统低地稻（Oryza sativa L.）的遗传多样性。
4. Assessment of nature and magnitude of genetic diversity based on DNA polymorphism with RAPD technique in traditional glutinous rice (Oryza sativa L.) of Assam. [J] . Shaptadvipa B., Sarma R. N. Asian Journal of Plant Sciences . 2009,第3期

机译：基于DNA多态性的RAPD技术对阿萨姆邦传统糯米（ L.）的遗传多样性进行评估。
5. Assessment of Nature and Magnitude of Genetic Diversity Based on DNA Polymorphism with RAPD Technique in Traditional Glutinous Rice ( Oryza sativa L.) of Assam [J] . B. Shaptadvipa, R.N. Sarma Asian Journal of Plant Sciences . 2009,第3期

机译：利用RAPD技术基于DNA多态性的阿萨姆传统糯稻（Oryza sativa L.）遗传多样性的性质和数量评估。
6. Genetics-Statistical Analysis of Traits Related to Grain in Rice (Oryza sativa L.) [C] . Mohammad H. Fotokian International Conference on Mathematical Biology . 2008

机译：水稻籽粒相关性的遗传学 - 统计分析（Oryza Sativa L.）
7. Development of genetic markers for blast resistance genes in rice (Oryza sativa L.) for use in marker assisted selection. [D] . Bormans, Concetta Ann. 2002

机译：水稻（Oryza sativa L.）稻瘟病抗性基因遗传标记的开发，用于标记辅助选择。
8. Genetic Diversity Assessment of Rarely Cultivated Traditional Indica Rice (Oryza sativa L.) Varieties [O] . T. Rekha, Kottackal Poulose Martin, V. B. Sreekumar, 2011

机译：珍稀传统Traditional稻（Oryza sativa L.）品种的遗传多样性评估
9. GENETIC DIVERSITY ANALYSIS OF RICE (Oryza sativa L.) LANDRACES THROUGH RAPD MARKERS [O] . M. S. Alam 2015

机译：通过RapD标记分析水稻（Oryza sativa L.）的遗传多样性

Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers

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