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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Genetic diversity assessment of Valeriana jatamansi Jones using microsatellites markers
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Genetic diversity assessment of Valeriana jatamansi Jones using microsatellites markers

机译：利用微卫星标记评估Valeriana jatamansi Jones的遗传多样性

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摘要

Valeriana jatamansi (family Valerianaceae) is a high-value medicinal plant used in traditional and modern medicine. In the present study, 25 populations (151 genotypes) of V. jatamansi were collected from Uttarakhand, India and investigated using nuclear and chloroplast SSR markers. Six nuclear and seven chloroplast polymorphic SSR primer pairs were used to evaluate genetic variability and population relatedness. These primer pairs have generated 59 fragments (26 nuclear, 33 chloroplast). The number of alleles per locus ranged from 3 to 7. Expected heterozygosity of the 25 V. jatamansi populations was 0.108-0.222 with a mean of 0.165 for nuclear SSR markers and 0.147-0.265 with a mean of 0.215 for chloroplast SSR markers. Based on AMOVA analysis, 6.0% (P = 0.001) of total genetic variation was found among populations. Nuclear SSR markers exhibited highest genetic diversity in samples collected from 1501 to 1800 m amsl altitudinal range and from pine forest habitat. In case of cpSSR, samples collected from 2101 to 2400 m amsl and grassland habitat exhibited highest diversity. These markers could be helpful in the identification and prioritization of genetically diverse populations/individuals for conservation and utilizing them in genetic improvement of V. jatamansi.
机译：Valeriana jatamansi（缬草科）是一种传统和现代医学中使用的高价值药用植物。在本研究中，从印度北阿坎德邦收集了25个种群（151个基因型）的贾塔曼斯弧菌，并使用核和叶绿体SSR标记进行了调查。使用六对核和七对叶绿体多态性SSR引物对评估遗传变异性和种群相关性。这些引物对已产生59个片段（26个核，33个叶绿体）。每个位点的等位基因数目范围为3至7。25个J.manata种群的预期杂合度为0.108-0.222，核SSR标记平均为0.165，叶绿体SSR标记平均为0.147-0.265。根据AMOVA分析，在人群中发现了6.0％（P = <0.001）的总遗传变异。在1501至1800 m amsl海拔范围和松林生境中采集的样品中，核SSR标记表现出最高的遗传多样性。在使用cpSSR的情况下，从2101至2400 m amsl和草地栖息地收集的样品表现出最高的多样性。这些标记可能有助于鉴定和区分遗传多样性种群/个体的优先次序，以进行保存并在V. jatamansi的遗传改良中利用它们。

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