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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators
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Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators

机译:胡萝卜(Daucus carota)愈伤组织培养中花青素产生的诱导和钙通道调节剂的参与。

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摘要

The effect of fungal elicitors and calcium-channel modulators on the anthocyanin production in callus cultures of Daucus carota was studied. It was found that the addition of fungal elicitors resulted in higher anthocyanin accumulation on the 3rd day of culture, but they resulted in lower levels of anthocyanin on the 21st day. Fungal elicitor treatment also affected growth of the cultures, as evident from the lower bio-mass accumulation under the elicitor treatments. Treatment with the calcium ionophore A23187 resulted in the enhancement of both growth and anthocyanin production. Ionophore treatment also resulted in higher Ca2+ ATPase activity. Addition of the calcium-channel blockers, verapamil and chlorpromazine, resulted in lowering of growth and anthocyanin production, suggesting the involvement of calcium in anthocyanin production by callus cultures of D. carota.
机译:研究了真菌激发剂和钙通道调节剂对胡萝卜鳞茎愈伤组织培养物中花青素生成的影响。发现在培养的第3天添加真菌引发剂会导致较高的花色素苷积聚,但在第21天会导致较低的花色素苷水平。真菌引发剂处理也影响培养物的生长,从引发剂处理下较低的生物量积累可以明显看出。用钙离子载体A23187处理导致生长和花青素生产均增强。离子载体处理还导致更高的Ca2 + ATPase活性。钙通道阻滞剂维拉帕米和氯丙嗪的添加会导致生长和花色苷的生成降低,这表明钙被D. carota愈伤组织培养物参与了花色苷的生成。

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