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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Characterization of defensin (Tfgd2) from Trigonella foenum-graecum.
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Characterization of defensin (Tfgd2) from Trigonella foenum-graecum.

机译：三角叶草的防御素（Tfgd2）的表征。

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摘要

Defensins are small cysteine-rich peptides with a size of 5-10 kDa, and some of them exhibit antifungal activity. We have cloned and sequenced a 219 bp coding region of the cDNA of a defensin from Trigonella foenumgraecum, designated as Tfgd2 using primers designed on the basis of a defensin, AlfAFP from Medicago sativa and reverse transcription-PCR. We have cloned the 701 bp genomic region of the defensin that comprised two exons and one long intron. The deduced amino acid sequence of Tfgd2 was similar to AlfAFP, except for two amino acid substitutions. It has 50% homology with the antifungal defensin Psd1 from Pisum sativum, whose NMR solution structure has been determined. The mature peptide has 45 amino acids, while the signal peptide comprised 27 amino acids. Southern analysis of the genomic DNA blot indicated that the defensins appear to be an oligo-gene family with at least two members in Trigonella. Purified peptide from Escherichia coli expression displayed inhibitory activity against broad-spectrum fungal pathogens, Rhizoctonia solani and Fusarium moniliforme [Gibberella fujikuroi].
机译：防御素是富含半胱氨酸的小肽，大小为5-10 kDa，其中一些具有抗真菌活性。我们已经使用基于防御素，紫花苜蓿的AlfAFP和逆转录PCR设计的引物，克隆并测序了来自三角藻的防御素的cDNA的219 bp编码区，并将其命名为Tfgd2。我们已经克隆了防御素的701 bp基因组区域，其中包含两个外显子和一个长内含子。推导的Tfgd2的氨基酸序列类似于AlfAFP，除了两个氨基酸取代。它与来自Pisum sativum的抗真菌防御素Psd1具有50％的同源性，后者的NMR溶液结构已确定。成熟的肽具有45个氨基酸，而信号肽包含27个氨基酸。对基因组DNA印迹的Southern分析表明防御素似乎是寡基因家族，在Trigonella中具有至少两个成员。从大肠杆菌表达中纯化的肽对广谱真菌病原体，茄状枯萎病菌和镰刀菌镰刀菌[Gibberella fujikuroi]具有抑制活性。

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