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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Characterizing the antioxidant activity of amla (Phyllanthus emblica)extract
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Characterizing the antioxidant activity of amla (Phyllanthus emblica)extract

机译：表征Amla（余甘子）提取物的抗氧化活性

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Amla is well-known for its rich vitamin C (ascorbic acid) and polyphenol contents. To assess its antioxidant activity, we examined aqueous amla extract for its ability to inhibit gamma -radiation-induced lipid peroxidation (LPO) in rat liver microsomes and superoxide dismutase (SOD) damage in rat liver mitochondria. For the LPO experiment, amla extract was added as its aqueous solution; and irradiation was carried out at different time intervals. The extent of LPO was measured in terms of thiobarbituric acid reactive substances. It was observed that the amla extract acts as a very good antioxidant against 7-radiation-induced LPO. Similarly, it was found to inhibit the damage to antioxidant enzyme SOD. The antioxidant activity of the amla extract was found to be both dose- and concentration-dependent. The amount of ascorbic acid in amla was standardized by HPLC and titrimetric methods and was found to be 3.25 to 4.5% w/w. However in microsomes containing this composition of pure ascorbic acid alone, no inhibition in LPO was observed. Cyclic voltammetry of the amla extract was carried out to estimate the ascorbic acid equivalents, which was found to be 9.4% w/w of amla. This value was found to be in agreement when compared with the reactivity of both amla and ascorbic acid towards ABTS(.-) radical, a stable free-radical. Based on these results it is concluded that amla is a more potent antioxidant than vitamin C.
机译：Amla以其丰富的维生素C（抗坏血酸）和多酚含量而闻名。为了评估其抗氧化活性，我们检查了amla提取物在大鼠肝微粒体中抑制伽马射线辐射诱导的脂质过氧化（LPO）和在大鼠肝线粒体中超氧化物歧化酶（SOD）损伤的能力。对于LPO实验，添加amla提取物作为其水溶液;并且以不同的时间间隔进行照射。 LPO的程度以硫代巴比妥酸反应性物质测量。观察到，amla提取物对7辐射诱导的LPO具有很好的抗氧化作用。类似地，发现抑制抗氧化酶SOD的破坏。发现安姆拉提取物的抗氧化活性是剂量和浓度依赖性的。通过HPLC和滴定法将amla中抗坏血酸的量标准化，发现为3.25-4.5％w / w。但是，在仅包含这种纯抗坏血酸组成的微粒体中，未观察到LPO的抑制作用。进行amla提取物的循环伏安法以估计抗坏血酸当量，发现抗坏血酸当量为amla的9.4％w / w。与amla和抗坏血酸对稳定的自由基ABTS（.-）自由基的反应性相比，发现该值是一致的。根据这些结果，可以得出结论，amla是比维生素C更有效的抗氧化剂。

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