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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Tracking of methanotrophs and their diversity in paddy soil: A molecular approach
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Tracking of methanotrophs and their diversity in paddy soil: A molecular approach

机译:稻田甲烷甲烷营养菌及其多样性的追踪:一种分子方法

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摘要

Methanotrophs, the inhabitants of irrigated rice soils, were monitored using molecular tools. Methanol dehydrogenase coded by mxaF locus has been used as the genus-specific locus. The presence of the locus has been demonstrated in DNA extracted from soil samples as well as in methanol utilizing isolates derived from those samples. Further, ARDRA pattern and cluster analysis revealed the following closely related genera - type-I methanotrophs Methylomonas and/or Methylocaldum in rhizosphere soil and coexistence of both type-I (Methylomonas and/or Methylocaldum) and type-II methanotrophs Methylobacterium and/or Methylocella in non-rhizosphere soil.
机译:使用分子工具监测灌溉水稻土壤中的甲烷营养菌。由mxaF基因座编码的甲醇脱氢酶已被用作属特异性基因座。在从土壤样品中提取的DNA以及利用从那些样品中提取的分离物的甲醇中已证明了该基因座的存在。此外,ARDRA模式和聚类分析揭示了以下密切相关的属-根际土壤中的I型甲烷营养菌和/或甲基本地化菌,以及I型(甲基化菌和/或Methylocaldum)和II型甲烷化菌和甲基细菌和/或甲基小球藻共存在非根际土壤中

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