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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane.
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Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane.

机译：克隆对病菌Colletotrichum falcatum产生拮抗作用的哈茨木霉部分内切酶基因cDNA。

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摘要

The saprophytic fungus T. harzianum (strain T5), which is antagonistic to C. falcatum [Glomerella tucumanensis], synthesized chitinases, i.e. N-acetyl- beta -D-glucosaminidase, 1,4- beta -D-N-N' chitobiosidase and 1,4- beta -D-N-N'-N" chitotriase, into the culture medium containing pathogenic cell wall or chitin. SDS-PAGE analysis showed the presence of 20- to 124-kDa extracellular proteins. The fungus produced a 97-kDa N-acetyl glucosaminidase in the medium amended with fungal cell wall or colloidal chitin. Chitobiase isoforms of 66, 56 and 50 kDa, as well as 66 and 50 kDa were detected in the media supplemented with fungal cell wall and colloidal chitin, respectively. The fungus also synthesized chitotriase isoforms of 66 and 50 kDa in the medium containing cell wall, and produced chitotriase isoforms of 50 kDa in the medium containing colloidal chitin. Chitinase isoforms of 66 and 50 kDa, which degraded both chitobiose and chitotriose, were isoforms of chitobiase and chitotriase that got separated at the same distance in SDS-PAGE. In an attempt to clone the endochitinase gene from T. harzianum T5, a partial cDNA of 246 bp (Genbank accession number AY762230) was obtained through RT-PCR. The deduced sequence showed high level of homology with chitinase sequences in the database..
机译：腐殖真菌哈茨木霉（T5菌株）与C. falcatum [Glomerella tucumanensis]拮抗，合成了几丁质酶，即N-乙酰基-β-D-氨基葡萄糖苷酶，1,4-β-DNN'壳聚糖酶和1,4。 -β-DN-N'-N“壳聚糖酶进入含有病原细胞壁或几丁质的培养基。SDS-PAGE分析显示存在20-124 kDa的胞外蛋白。真菌产生97-kDa的N-乙酰基在用真菌细胞壁或胶体几丁质修饰的培养基中添加了氨基葡萄糖苷酶，在添加了真菌细胞壁和胶体几丁质的培养基中分别检测到66、56和50 kDa以及66和50 kDa的壳聚糖酶同工型。含有细胞壁的培养基中的壳聚糖酶同工型分别为66和50 kDa，在含有胶体甲壳质的培养基中产生的壳聚糖酶同工型为50 kDa，降解壳聚糖和壳三糖的壳聚糖酶同工酶分别为66和50 kDa的同工型。 t在SDS-PAGE中以相同的距离分离。为了从哈茨木霉T5克隆内切几丁质酶基因，通过RT-PCR获得了246bp的部分cDNA（Genbank登录号AY762230）。推导的序列与数据库中的几丁质酶序列具有高度的同源性。

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