Biochemical and molecular evidence of azadirachtin binding to insect actins

Anuradha A.; Annadurai R. S.

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Biochemical and molecular evidence of azadirachtin binding to insect actins

机译：印za素与昆虫肌动蛋白结合的生化和分子证据

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Azadirachtin, a neem seed terpenoid is effective against serious lepidopteran crop pests like Plutella xylostella and Spodoptera litura. The mode of action of azadirachtin and its cellular target in insects has been largely unexplored, although physiological and morphological defects have been documented. Understanding the mechanism of action of this complex natural molecule at the cellular level is crucial to define safety and entomo-specific affinity. Actin has been recently indicated as a putative target of azadirachtin in vivo and in silico using Drosophila melanogaster. In this communication, the target of azadirachtin in Plutella and Drosophila has been validated by in vitro polymerization assays of actin and its mRNA transcript profiles of Act5C, a ubiquitous cytoplasmic and Act57B, a muscle-specific isoform. Further, phalloidin labelling of Spodoptera haemocytes identifies the exact passage of azadirachtin on the highly proliferating developmental tissues.

机译：印za种子萜类化合物印za素对付严重的鳞翅目作物害虫，如小菜蛾和斜纹夜蛾有效。印a素和其细胞靶标在昆虫中的作用方式虽然尚未发现生理和形态上的缺陷，但在很大程度上尚未得到研究。了解这种复杂的天然分子在细胞水平上的作用机理对于定义安全性和特定于昆虫的亲和力至关重要。最近已经表明，使用果蝇（Drosophila melanogaster），肌动蛋白是印za素在体内和计算机上的印target素的推定靶标。在这种交流中，印by素在小菜蛾和果蝇中的靶点已通过肌动蛋白的体外聚合测定法和遍在细胞质的Act5C和肌肉特异性同种型Act57B的mRNA转录谱进行了验证。此外，斜纹夜蛾血细胞的鬼笔环肽标记可鉴定印za素可能在高度增殖的发育组织上的确切通道。

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《Current Science: A Fortnightly Journal of Research》 |2008年第11期|共6页
作者
Anuradha A.; Annadurai R. S.;
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正文语种 eng
中图分类自然科学现状及发展;
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molecular evidence; biochemical evidence; entomo-specific affinity; muscle-specific isoform; developmental tissue;

机译：分子证据;生化证据;昆虫特异性亲和力;肌肉特异性同工型;发育组织;

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1. Biochemical and molecular evidence of azadirachtin binding to insect actins [J] . Anuradha A., Annadurai R. S. Current Science: A Fortnightly Journal of Research . 2008,第11期

机译：印za素与昆虫肌动蛋白结合的生化和分子证据
2. Cofilin and gelsolin segment-1: Molecular dynamics simulation and biochemical analysis predict a similar actin binding mode [J] . Wriggers W., Azuma T., Marks PW., Journal of Molecular Biology . 1998,第5期

机译：Cofilin和凝溶胶蛋白segment-1：分子动力学模拟和生化分析预测相似的肌动蛋白结合模式
3. Actin-binding proteins as molecular targets to modulate hepatic stellate cell proliferation. Focus on "MARCKS actin-binding capacity mediates actin filament assembly during mitosis in human hepatic stellate cells" [J] . Gemma Odena1 Ramon Bataller American Journal of Physiology . 2012,第2aPta1期

机译：肌动蛋白结合蛋白作为调节肝星状细胞增殖的分子靶标。专注于“ MARCKS肌动蛋白结合能力在人肝星状细胞的有丝分裂过程中介导肌动蛋白丝组装”
4. The efficacy of flufenoxuron and azadirachtin against mixed mite and insect populations in small bins of wheat [C] . D.A. Collins International Working Conference on Stored Product Protection . 2003

机译：Flyfenoxuron和Azadirachtin对小麦小箱混合螨和昆虫种群的疗效
5. Evidence for a role of actin-binding in the regulatory effects of cardiac myosin binding protein c. [D] . Bezold, Kristina L. 2013

机译：肌动蛋白结合在心肌肌球蛋白结合蛋白c的调节作用中的作用的证据。
6. Molecular analysis of insertion/deletion mutations in protein 4.1 in elliptocytosis. I. Biochemical identification of rearrangements in the spectrin/actin binding domain and functional characterizations. [O] . S L Marchesi, J Conboy, P Agre, 1990

机译：卵白细胞增多症中蛋白4.1的插入/缺失突变的分子分析。 I.血影蛋白/肌动蛋白结合域中重排的生化鉴定和功能表征。
7. Molecular analysis of insertion/deletion mutations in protein 4.1 in elliptocytosis. I. Biochemical identification of rearrangements in the spectrin/actin binding domain and functional characterizations. [O] . Marchesi, S L, Conboy, J, Agre, P, 1990

机译：卵白细胞增多症中蛋白4.1的插入/缺失突变的分子分析。 I.血影蛋白/肌动蛋白结合域中重排的生化鉴定和功能表征。

Biochemical and molecular evidence of azadirachtin binding to insect actins

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