• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Simultaneous detection of TNOS and P35S in transgenic soybean based on magnetic bicolor fluorescent probes
【24h】

Simultaneous detection of TNOS and P35S in transgenic soybean based on magnetic bicolor fluorescent probes

机译：基于磁性双子荧光探针的转基因大豆同时检测TNOS和P35s

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A magnetic-separation-dual-targets fluorescent biosensor was fabricated to detect terminator nopaline synthase (TNOS) and promoter of cauliflower mosaic virus 35s (P35S) in transgenic soybean based on incorporation of bicolor CdTe quantum dots carried by silica nanospheres. In this protocol, the fixed probes for TNOS or P35S were magnetized firstly with Fe3O4@Au magnetic nanosphere by Au-S covalent bonding to achieve magnetized probes. Meanwhile, the capture probes for TNOS or P35S were functionalized with green or red fluorescent microspheres respectively to obtain fluorescently-labeled probes, which could emit relative strong green or red fluorescent signal. Two terminals of TNOS or P35S were recognized by magnetized probes and fluorescently-labeled probes respectively to form the sandwiched structures in the process of biosensor development subsequently, and it was separated by a magnet instantly. The fluorescence intensities of remnant supernatant were measured and analyzed accordingly to achieve simultaneous detection of TNOS and P35S. This biosensor exhibited a good dynamic range, low limit of detection and excellent selectivity in detecting transgenic soybean.
机译：基于二氧化硅纳米球掺入，制备磁性分离 - 双靶荧光生物传感器以检测转基因大豆中的终止剂Nopaline合成酶（TNOS）和Cafiflower Mosaic病毒病毒35s（P35s）的启动子。在该方案中，通过AU-S共价键合，首先用Fe3O4磁性纳米磁纳米磁纳米磁性纳米磁性纳米磁性纳米磁性纳米磁性纳米磁性纳米级固定探针。同时，TNO或P35s的捕获探针分别用绿色或红色荧光微球官能化，得到荧光标记的探针，其可以发射相对强大的绿色或红色荧光信号。通过磁化探针和荧光标记的探针识别出两端的TNO或P35s，以随后在生物传感器显影过程中形成夹心结构，并且它立即用磁铁分离。测量并分析残余上清液的荧光强度以实现TNO和P35s的同时检测。这种生物传感器表现出良好的动态范围，检测和检测转基因大豆的优异选择性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号