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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Automated NMR assignments of proteins for high throughput structure determination: TATAPRO II
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Automated NMR assignments of proteins for high throughput structure determination: TATAPRO II

机译:用于高通量结构测定的蛋白质的自动NMR分配:TATAPRO II

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摘要

TATAPRO (Tracked Automated Assignments in Proteins), a novel algorithm for automated NMR assignments in proteins is presented to aid in high throughput protein structure determination. In this version (TATAPRO 11), the 20 amino acid residues are classified into nine distinct categories based on their characteristic C-13(alpha) and C-13(beta) chemical shifts derived statistically using a database of similar to 100,000 shifts. Further, in the current version, the N- and C-terminal residues of an assigned polypeptide stretch are retained during the course of resonance assignments. This results in faster execution time of the program, increased efficiency and greater robustness towards missing peaks.
机译:TATAPRO(蛋白质中的跟踪自动分配)是一种用于蛋白质中NMR自动分配的新颖算法,可帮助确定高通量蛋白质结构。在此版本(TATAPRO 11)中,根据其特征性C-13α和C-13β化学位移(使用近似100,000个位移的数据库统计得出),将20个氨基酸残基分为9个不同的类别。此外,在当前版本中,在共振分配的过程中保留了指定的多肽片段的N-和C-末端残基。这样可以缩短程序的执行时间,提高效率,并能更好地防止丢失峰。

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