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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >A rapid method for measuring olfactory responses of Drosophila larva
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A rapid method for measuring olfactory responses of Drosophila larva

机译：一种测量果蝇幼虫嗅觉反应的快速方法

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摘要

The olfactory responses of Drosophila larvae are measured by following its chemotactic movements on a petri dish. Several variants of this test have been employed. In hte commonly used version of the experiment, diluted odorant is placed near the dege of the dish and a spot of the diluent diametrically opposite. After 5 min, the larvae on the two halves of the dish, the odour side (0) and the control side (C) are counted, and the response index is computed as 0 - C/ 0 + C. This method of measuring olfactory response suffers from certain disadvantages. The test is slow; since the odour spreads on the dish by diffusion, it takes a few minutes for the larvae on the farther side of the odour source to respond. On the other hand, the larvae near the source respond early and, if the concentration of the odorant is high, they become desensitized and wander-off. We describe here a modification of the larval test, which enables us to assess the attraction response rapidly by measuring the initial rate of entry in a zone around the odour source.
机译：果蝇幼虫的嗅觉反应通过在培养皿上的趋化运动来测量。已经使用了该测试的几种变体。在该实验的常用版本中，将稀释的加味剂放置在培养皿的边缘附近，并且稀释剂的位置完全相反。 5分钟后，对两半盘上的幼虫（气味侧（0）和对照侧（C））进行计数，并将响应指数计算为0-C / 0 +C。这种测量嗅觉的方法反应具有某些缺点。测试很慢;由于气味是通过扩散扩散到盘子上的，因此气味源另一侧的幼虫需要几分钟的时间才能做出反应。另一方面，源附近的幼虫反应较早，如果气味剂的浓度很高，它们就会变得脱敏并游走。我们在这里描述了幼虫测试的一种改进，它使我们能够通过测量气味源周围区域的初始进入速率来快速评估吸引力响应。

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