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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Green hairy root cultures of Solanum khasianum Clarke - a new route to in vitro solasodine production
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Green hairy root cultures of Solanum khasianum Clarke - a new route to in vitro solasodine production

机译：Solanum khasianum Clarke的绿色毛状根培养物-体外生产solaso​​dine的新途径

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摘要

Solasodine is recognized as a potential alternative to diosgonin. Both share the characteristic that they can be converted to 16-dehydro-pregnenolone acetate; the first step in steroid synthesis. Effort has been ongoing in the production of solasodine using cell and lately hairy root cultures of various Solanum species. As seen in our pilot experiments, some of the hairy root lines of Solanum khasianum Clarke show enhancement of solasodine production compared to non-transformed roots. Light and temperature as physical factors of growth are found to have an important role in controlling greening, growth and secondary product formation in these hairy root cultures. This is similar to the greening observed in tubers of Solanum tuberosum (potato), leading to stimulation of solanine production. Enrichment of the nutrient medium with CO2 further increases growth and secondary metabolite production in the hairy root cultures of S. khasianum. Thus by manipulating environmental and nutritional conditions, solasodine production can be maximized in hairy root cultures of S. khasianum.
机译：Solaso​​dine被认为是薯di中潜在的替代品。两者都具有可以转化为16-脱氢-孕烯醇酮乙酸盐的特征。类固醇合成的第一步。使用各种茄属物种的细胞和近毛状根培养物一直在努力生产索拉索定。从我们的先导实验中可以看出，与未转化的根相比，茄果茄的一些多毛根系显示出solaso​​dine的产量增加。发现光和温度是生长的物理因素，在控制这些毛状根培养物中的绿化，生长和次要产品形成中具有重要作用。这类似于在马铃薯（马铃薯）块茎中观察到的绿化，从而刺激了茄碱的产生。用二氧化碳富集营养培养基可进一步增加kha.num。Shamsianum毛状根培养物中的生长和次生代谢产物的产生。因此，通过控制环境和营养条件，可在khasananum的毛状根培养物中使索拉索定的产量最大化。

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