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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety, ADT39.
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Regeneration and Agrobacterium-mediated transformation of a popular indica rice variety, ADT39.

机译：流行的rice稻品种ADT39的再生和农杆菌介导的转化。

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摘要

A simple and efficient protocol for the regeneration and Agrobacterium-mediated transformation of an agronomically useful indica rice variety, ADT39 has been standardized. Initiation of callusing and its subculture were best achieved in MS medium supplemented with 300 mg casein enzymatic hydrolysate/litre. The best callusing and regeneration responses were observed at 2 mg 2,4-D and 1.5 mg benzyladenine/litre. An optimum transformation efficiency of 22.2% was obtained using high concentrations of augmentin as a bacteriostatic agent for a short period to inhibit the growth/persistence of Agrobacterium, without compromising the regeneration potential of the tissue. Using a viral promoter-driven GUS reporter gene, morphologically normal fertile plants were obtained. Molecular analysis of the above transgenic plants indicated several independent, single-copy transgene insertion events. Expression of the reporter gene was detected in the above plants. Mendelian inheritance of the transgene in the progeny was also observed.
机译：ADT39是用于农学上有用的ica稻品种的再生和农杆菌介导的转化的一种简单有效的方法，已经标准化。在补充了300 mg酪蛋白酶解液/升的MS培养基中，最佳地实现愈伤组织的启动及其继代培养。在2 mg 2,4-D和1.5 mg苄腺嘌呤/升下观察到最佳的愈伤组织和再生响应。使用高浓度的增强素作为抑菌剂，在短期内抑制农杆菌的生长/持久性而不损害组织的再生潜力，可获得22.2％的最佳转化效率。使用病毒启动子驱动的GUS报道基因，获得了形态正常的可育植物。上述转基因植物的分子分析表明了几种独立的单拷贝转基因插入事件。在上述植物中检测到报告基因的表达。还观察到子代中转基因的孟德尔遗传。

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